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Cytoplasmic Trna 2-Thiolation Protein 1 (NCS6) Yarrowia Lipolytica Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1349274

MBS1349274 is a recombinant protein targeting NCS6. Also known as Recombinant Yarrowia lipolytica Cytoplasmic tRNA 2-thiolation protein 1 (NCS6). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

$1085 / 0.02 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Cytoplasmic tRNA 2-thiolation protein 1 (NCS6), Recombinant Protein
Full Product Name
Recombinant Yarrowia lipolytica Cytoplasmic tRNA 2-thiolation protein 1 (NCS6)
Product Gene Name
Product Synonym Gene Name
NCS6
Sequence
MLCVLCECRK AMLKRPKTLQ PICKPCFYNV FETEIHNTIV ESNLFFPGER VAIGASGGKD STVLAHVMKT LNERYNYGVD FVLLSIDEGI HGYRDDSLET VKRNKVQYDM DLEVVSYSEL YGWSMDQIVA QIGNKNNCTY CGVFRRQALD RGSAKLGIAH IVTGHNADDM AETVLMNLLR GDTARLDRCT ELVTGSDDSP VKRSKPLKYA YEKEIVLYAH YKKLDYFSTE CTYSPEAFRG TARTLIKNLE AIRPSTIIDI IHSGEAFVLK KKKEKKGKGS VVVKAKVEEK PVPSGGCSVP LAFDSSLGLS NRCTKCGYLS HNAVCKACVL LEGLNAGRAK MQIEGDSADG AAKNIKTLEK LALSI
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
Molecular Weight
40,376 Da
Sequence Positions
1-365, Full length
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for Q6C8R5
Species
Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of NCS6 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NCS6. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
YALI0D17600p
NCBI Official Symbol
YALI0_D17600g
UniProt Protein Name
Cytoplasmic tRNA 2-thiolation protein 1
UniProt Synonym Protein Names
Cytoplasmic tRNA adenylyltransferase 1
UniProt Gene Name
NCS6
NCBI Protein Information
YALI0D17600p

UniProt Comments for NCS6

Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm5 tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with NCS6 recombinant protein

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