ABSCISIC ACID-Insensitive 5 (ABI5) Recombinant Protein

Participates in ABA-regulated gene expression during seed development and subsequent vegetative stage by acting as the major mediator of ABA repression of growth. Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the 1 and 6 genes promoters. Can also trans-activate its own promoter, suggesting that it is autoregulated. Plays a role in sugar-mediated senescence

The Arabidopsis abscisic acid response gene ABI5 encodes a basic leucine zipper transcription factor.Finkelstein R.R., Lynch T.J.Plant Cell 12:599-609(2000) Arabidopsis ABI5 subfamily members have distinct DNA-binding and transcriptional activities.Kim S.Y., Ma J., Perret P., Li Z., Thomas T.L.Plant Physiol. 130:688-697(2002) Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.Nature 402:761-768(1999) The Arabidopsis Information Resource (TAIR) Arabidopsis ORF Clones.Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R. A null mutation in a bZIP factor confers ABA-insensitivity in Arabidopsis thaliana.Lopez-Molina L., Chua N.H.Plant Cell Physiol. 41:541-547(2000) Physical interactions between ABA response loci of Arabidopsis.Nakamura S., Lynch T.J., Finkelstein R.R.Plant J. 26:627-635(2001) A postgermination developmental arrest checkpoint is mediated by abscisic acid and requires the ABI5 transcription factor in Arabidopsis.Lopez-Molina L., Mongrand S., Chua N.-H.Proc. Natl. Acad. Sci. U.S.A. 98:4782-4787(2001) The homologous ABI5 and EEL transcription factors function antagonistically to fine-tune gene expression during late embryogenesis.Bensmihen S., Rippa S., Lambert G., Jublot D., Pautot V., Granier F., Giraudat J., Parcy F.Plant Cell 14:1391-1403(2002) Regulation of Arabidopsis thaliana Em genes role of ABI5.Carles C., Bies-Etheve N., Aspart L., Leon-Kloosterziel K.M., Koornneef M., Echeverria M., Delseny M.Plant J. 30:373-383(2002) ABI5 acts downstream of ABI3 to execute an ABA-dependent growth arrest during germination.Lopez-Molina L., Mongrand S., McLachlin D.T., Chait B.T., Chua N.-H.Plant J. 32:317-328(2002) Regulation and role of the Arabidopsis abscisic acid-insensitive 5 gene in abscisic acid, sugar, and stress response.Brocard I.M., Lynch T.J., Finkelstein R.R.Plant Physiol. 129:1533-1543(2002) Mitogen-activated protein kinase signaling in postgermination arrest of development by abscisic acid.Lu C., Han M.-H., Guevara-Garcia A., Fedoroff N.V.Proc. Natl. Acad. Sci. U.S.A. 99:15812-15817(2002) bZIP transcription factors in Arabidopsis.Jakoby M., Weisshaar B., Droege-Laser W., Vicente-Carbajosa J., Tiedemann J., Kroj T., Parcy F.Trends Plant Sci. 7:106-111(2002) AFP is a novel negative regulator of ABA signaling that promotes ABI5 protein degradation.Lopez-Molina L., Mongrand S., Kinoshita N., Chua N.-H.Genes Dev. 17:410-418(2003) The pleiotropic role of the 26S proteasome subunit RPN10 in Arabidopsis growth and development supports a substrate-specific function in abscisic acid signaling.Smalle J., Kurepa J., Yang P., Emborg T.J., Babiychuk E., Kushnir S., Vierstra R.D.Plant Cell 15:965-980(2003) Three genes that affect sugar sensing (abscisic acid insensitive 4, abscisic acid insensitive 5, and constitutive triple response 1) are differentially regulated by glucose in Arabidopsis.Arroyo A., Bossi F., Finkelstein R.R., Leon P.Plant Physiol. 133:231-242(2003) Interactions of abscisic acid and sugar signalling in the regulation of leaf senescence.Pourtau N., Mares M., Purdy S., Quentin N., Rueel A., Wingler A.Planta 219:765-772(2004) Redundant and distinct functions of the ABA response loci ABA-INSENSITIVE(ABI) 5 and ABRE-BINDING FACTOR (ABF) 3.Finkelstein R.R., Gampala S.S., Lynch T.J., Thomas T.L., Rock C.D.Plant Mol. Biol. 59:253-267(2005) KEEP ON GOING, a RING E3 ligase essential for Arabidopsis growth and development, is involved in abscisic acid signaling.Stone S.L., Williams L.A., Farmer L.M., Vierstra R.D., Callis J.Plant Cell 18:3415-3428(2006) Transcriptional regulation of ABI3- and ABA-responsive genes including RD29B and RD29A in seeds, germinating embryos, and seedlings of Arabidopsis.Nakashima K., Fujita Y., Katsura K., Maruyama K., Narusaka Y., Seki M., Shinozaki K., Yamaguchi-Shinozaki K.Plant Mol. Biol. 60:51-68(2006) Identification of two protein kinases required for abscisic acid regulation of seed germination, root growth, and gene expression in Arabidopsis.Fujii H., Verslues P.E., Zhu J.-K.Plant Cell 19:485-494(2007) A small plant-specific protein family of ABI five binding proteins (AFPs) regulates stress response in germinating Arabidopsis seeds and seedlings.Garcia M.E., Lynch T.J., Peeters J., Snowden C., Finkelstein R.R.Plant Mol. Biol. 67:643-658(2008) Integration of light and abscisic acid signaling during seed germination and early seedling development.Chen H., Zhang J., Neff M.M., Hong S.-W., Zhang H., Deng X.-W., Xiong L.Proc. Natl. Acad. Sci. U.S.A. 105:4495-4500(2008) Sumoylation of ABI5 by the Arabidopsis SUMO E3 ligase SIZ1 negatively regulates abscisic acid signaling.Miura K., Lee J., Jin J.B., Yoo C.Y., Miura T., Hasegawa P.M.Proc. Natl. Acad. Sci. U.S.A. 106:5418-5423(2009) The Arabidopsis mediator subunit MED25 differentially regulates jasmonate and abscisic acid signaling through interacting with the MYC2 and ABI5 transcription factors.Chen R., Jiang H., Li L., Zhai Q., Qi L., Zhou W., Liu X., Li H., Zheng W., Sun J., Li C.Plant Cell 24:2898-2916(2012)

Small volumes of ABI5 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS1354042 is a recombinant protein targeting ABI5. Also known as Recombinant Arabidopsis thaliana Protein ABSCISIC ACID-INSENSITIVE 5. Host: E Coli or Yeast or Baculovirus or Mammalian Cell.