Rabbit Immunoglobulin G ELISA Kit for General

Intended Uses: This rabbit Immunoglobulin G (IgG) antigen assay is intended for the quantitative determination of total rabbit IgG antigen in serum, plasma, hybridoma cell supernatants, ascites or other biological fluids. The assay does not distinguish IgG subclasses.

Principle of the Assay: Rabbit IgG will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, horseradish peroxidase labeled polyclonal anti-rabbit IgG antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of rabbit IgG. Color development is proportional to the concentration of IgG in the samples.

Background: IgG is the most abundant immunoglobulin in serum and is predominately involved in the secondary immune response. The IgG subclasses are designated 1, 2, 3 and 4 based on their relative prevalence in human serum.

Small volumes of Rabbit Immunoglobulin G elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS135512 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Rabbit Immunoglobulin G, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Rabbit Immunoglobulin G. The ELISA analytical biochemical technique of the MBS135512 kit is based on Rabbit Immunoglobulin G antibody-Rabbit Immunoglobulin G antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Rabbit Immunoglobulin G antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Rabbit Immunoglobulin G. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).