Vitronectin Total Antigen Assay (VTN) ELISA Kit for General

Principle of the Assay: Mouse vitronectin will bind to the affinity purified capture antibody coated on the microtiter plate. Free and ligand bound enzyme will react with the antibody on the plate. After appropriate washing steps, biotinylated anti-mouse vitronectin primary antibody binds to the captured protein. Excess antibody is washed away and bound polyclonal antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of mouse vitronectin. The amount of color development is directly proportional to the concentration of total vitronectin in the sample.
Background: Vitronectin is an abundant plasma glycoprotein that helps regulate coagulation, fibrinolysis, complement activation, and cell adhesion [1,3,7]. Vitronectin binds to glycosaminoglycans, collagen, plasminogen and urokinase receptors. It also may control the clearance of vascular thrombi by binding and stabilizing PAI-1. In binding PAI-1, it extends the lifetime of active PAI-1 [4,5]. Vitronectin may also be involved in the regulation of bone metabolism [2].

Small volumes of VTN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS135526 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the vitronectin total antigen assay (VTN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing VTN. The ELISA analytical biochemical technique of the MBS135526 kit is based on VTN antibody-VTN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect VTN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, VTN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).