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Factor X Total Antigen Assay (F10) ELISA Kit for General

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS135527

Intended Uses: This mouse Factor X total assay is for the quantitative determination of total Factor X and Xa in biological fluids. For research use only.Principle of the Assay: Mouse Factor X will bind to the affinity purified capture antibody coated on the… More details

$850 / 1 Kit +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Factor X total antigen assay (F10), ELISA Kit
Full Product Name
Mouse Factor X total antigen assay ELISA Kit
Product Gene Name
F10 elisa kit
Species Reactivity
General
Samples
Biological fluids
Sensitivity
The minimum detectable dose (MDD) was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates (range OD450: 0.069-0.075) and calculating the corresponding concentration. The MDD was 0.146 ng/ml.
Form/Format
Complete Kit
Specificity
These studies are currently in progress. Please contact us for more information.
Preparation and Storage
Store at 4 degree C. Shelf Life: 12 months from manufacture, see label for expiration date
Inter-assay Precision
These studies are currently in progress. Please contact us for more information.
Intra-assay Precision
These studies are currently in progress. Please contact us for more information.
Molecular Weight
54,018 Da
Chromosome Location
Chromosome: 8; NC_000074.6 (13037308..13056676). Location: 8 A1.1; 8 5.73 cM
3D Structure
ModBase 3D Structure for O88947

Product Categories/Family for F10 elisa kit

Factor X; Mouse Factor X ELISA Kits

Typical Testing Data/Standard Curve (for reference only) of F10 elisa kit

General F10 ELISA Kit – Cat# MBS135527

Other Notes

Small volumes of F10 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for F10 purchase

MBS135527 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Factor X total antigen assay (F10) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing F10. The ELISA analytical biochemical technique of the MBS135527 kit is based on F10 antibody-F10 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect F10 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, F10. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for F10. It may not necessarily be applicable to this product.

NCBI GI #
110625994
NCBI GeneID
14058
NCBI Accession #
NP_031998.3
UniProt Primary Accession #
O88947
UniProt Secondary Accession #
O54740; Q99L32
UniProt Related Accession #
O88947
GenBank Nucleotide #
NM_007972.4
NCBI Official Full Name
coagulation factor X isoform 2 preproprotein
NCBI Official Synonym Full Names
coagulation factor X
NCBI Official Symbol
F10
NCBI Official Synonym Symbols
fX; Cf10
UniProt Protein Name
Coagulation factor X
UniProt Synonym Protein Names
Stuart factor
Protein Family
Coagulation factor
UniProt Gene Name
F10
UniProt Entry Name
FA10_MOUSE
NCBI Protein Information
coagulation factor X; stuart factor

NCBI Summary for F10

This gene encodes factor X, a component of both the intrinsic and extrinsic blood coagulation pathways. The encoded protein is a zymogen that undergoes further processing in a vitamin K-dependent manner to generate mature factor X, a heterodimer comprised of disulfide-linked heavy and light chains. The mature factor X is proteolytically activated either by factor IXa (intrinsic pathway) or factor VIIa (extrinsic pathway) to form factor Xa serine endopeptidase. Activated factor Xa catalyzes the conversion of prothrombin to thrombin. A complete lack of the encoded protein is fatal to mice. A severe deficiency of the encoded protein in mice causes age-dependent iron deposition and cardiac fibrosis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]
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UniProt Comments for F10

F10: Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. Defects in F10 are the cause of factor X deficiency (FA10D). A hemorrhagic disease with variable presentation. Affected individuals can manifest prolonged nasal and mucosal hemorrhage, menorrhagia, hematuria, and occasionally hemarthrosis. Some patients do not have clinical bleeding diathesis. Belongs to the peptidase S1 family.

Protein type: EC 3.4.21.6; Secreted; Lipid-binding; Motility/polarity/chemotaxis; Secreted, signal peptide; Protease

Cellular Component: intracellular membrane-bound organelle; membrane; extracellular region

Molecular Function: peptidase activity; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; phospholipid binding; calcium ion binding; catalytic activity

Biological Process: positive regulation of protein kinase B signaling cascade; hemostasis; proteolysis; blood coagulation
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Research Articles on F10

1. Differential effects of murine and human factor X on adenovirus transduction via cell-surface heparan sulfate.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with F10 elisa kit

 Products by Pathway  Pathway Diagram
 Blood Clotting Cascade Pathway antibodies  Blood Clotting Cascade Pathway Diagram
 Common Pathway antibodies  Common Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Extrinsic Pathway antibodies  Extrinsic Pathway Diagram
 Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies  Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
 Gamma-carboxylation Of Protein Precursors Pathway antibodies  Gamma-carboxylation Of Protein Precursors Pathway Diagram
 Gamma-carboxylation, Transport, And Amino-terminal Cleavage Of Proteins Pathway antibodies  Gamma-carboxylation, Transport, And Amino-terminal Cleavage Of Proteins Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram

Diseases associated with F10 elisa kit

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1018 publications with F10 and Neoplasms
 Lung Neoplasms Antibodies  >294 publications with F10 and Lung Neoplasms
 Drug Toxicity Antibodies  >73 publications with F10 and Drug Toxicity
 Nervous System Diseases Antibodies  >73 publications with F10 and Nervous System Diseases
 Necrosis Antibodies  >60 publications with F10 and Necrosis
 Liver Neoplasms Antibodies  >40 publications with F10 and Liver Neoplasms
 Brain Diseases Antibodies  >34 publications with F10 and Brain Diseases
 Liver Diseases Antibodies  >32 publications with F10 and Liver Diseases
 Inflammation Antibodies  >32 publications with F10 and Inflammation
 Liver Neoplasms, Experimental Antibodies  >30 publications with F10 and Liver Neoplasms, Experimental

Organs/Tissues associated with F10 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >431 publications with F10 and Blood
 Lung Antibodies  >409 publications with F10 and Lung
 Skin Antibodies  >127 publications with F10 and Skin
 Liver Antibodies  >86 publications with F10 and Liver
 Embryonic Tissue Antibodies  >52 publications with F10 and Embryonic Tissue
 Bone Marrow Antibodies  >31 publications with F10 and Bone Marrow
 Intestine Antibodies  >14 publications with F10 and Intestine
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