Tpa Functional Assay (PLAT) ELISA Kit for General

Background: Tissue plasminogen activator (tPA) is a serine protease that converts plasminogen to plasmin in the blood fibrinolytic system [1,2,3,9). It also plays an important role in the nervous syslem, incl uding the processes of neuronal migration, neurite outgrowth, and neuronal plasticity [1,2,4,7, 10). t PA has been suggested to have a role in several neuropathological conditions such as cerebral ischemia, seizures, and demyelinating diseases [1,3,5).

Principle of the Assay: Functionally active mouset PA will bind to the biotinylated human PAI-1 coated on the microtiter plate. Only free active enzyme will react with the PAI-1 on the plate. A standard calibration curve is prepared using dilutions of tPA along with the samples to be measured. After appropriate washing steps, monoclonal anti-mouset PA primary antibody binds to the captured enzyme. Excess antibody is washed away and bound monoclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. Following an additional washing step, TMB is used for color development at 450nm. The amount of color development is directly proportional to the concent ration of active tPA in the sample.

Intended Uses: This mouse tPA activi ty assay is intended for the quantitative determi nation of active tissue plasm inogen activator in mous" plasma and other biological f luids. For research use only.

Small volumes of PLAT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS135539 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the tPA functional assay (PLAT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PLAT. The ELISA analytical biochemical technique of the MBS135539 kit is based on PLAT antibody-PLAT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PLAT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PLAT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).