Russells Viper Venom Factor V Activator (SVSP) Protease

Name: SVSP Protease; MyBioSource
Brand: MyBioSource
SKU: MBS135607
Price: 1000 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS135607

Purified from Russells Viper Venom. Serine protease that activates single chain Factor V to two chain active Va. This protease is inhibited by diisopropylfluorophosphate (DFP). RVV-V is a single chain glycoprotein and is highly pure and homogenous by SDS- More details

$1000 / 1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Russells Viper Venom Factor V Activator (SVSP), Protease

Full Product Name

Russells Viper Venom Factor V Activator

Product Gene Name

SVSP protease

Host

Snake venom

Form/Format

Liquid

Purity/Purification

>95% by SDS-PAGE analysis

Concentration

10.0 mg/ml (lot specific)

Preparation and Storage

Store at -20 degree C. Shelf Life: 12 months from delivery

Molecular Weight

27,200

3D Structure

ModBase 3D Structure for P18964

Buffer

50% Glycerol

Extinction Coefficient

1.5

Product Categories/Family for SVSP protease

Snake Venom Proteases; Russells Viper Proteases

Other Notes

Small volumes of SVSP protease vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SVSP purchase

MBS135607 is a protease targeting SVSP. Also known as Russells Viper Venom Factor V Activator. Host: Snake venom.

Additional Details

NCBI/Uniprot data below describe general gene information for SVSP. It may not necessarily be applicable to this product.

NCBI GI #

134129

NCBI Accession #

P18964.1

UniProt Primary Accession #

P18964

NCBI Official Full Name

Factor V activator RVV-V alpha

UniProt Protein Name

Factor V activator RVV-V alpha

UniProt Synonym Protein Names

Russel's viper venom FV activator alpha; RVV-V alpha; Snake venom serine protease

UniProt Gene Name

RVV-V alpha

UniProt Synonym Gene Names

SVSP

UniProt Entry Name

VSPA_DABSI

UniProt Comments for SVSP

Function: Venom serine protease that activates factor V (F5) in a calcium-independent manner. It cleaves the Arg(1545)-Ser(1546) linkage in the human factor V molecule. Induces the coagulation of mammalian plasma. Ref.1 Ref.2

Catalytic activity: Fully activates human clotting factor V by a single cleavage at the 1545-Trp-Tyr-Leu-Arg-|-Ser-Asn-Asn-Gly-1552 bond. Cattle, but not rabbit, factor V is cleaved, and no other proteins of the clotting system are attacked. Esterase activity is observed on Bz-Arg-OEt and Tos-Arg-OMe, and amidase activity on Phe-pipecolyl-Arg-NHPhNO2.

Enzyme regulation: Inhibited by D-Phe-Pro-Arg-chloromethyl ketone (FPRCK) (97%), PMSF (76%), and benzamidine (50%). Is not inhibited by BPTI, antithrombin and EDTA. Ref.2

Subunit structure: Monomer.

Subcellular location: Secreted.

Tissue specificity: Expressed by the venom gland.

Miscellaneous: There are three isoproteins of RVV-V, designated RVV-V alpha, V-beta, and V-gamma.Has no proteolytic effect on factor VIII, factor XIII, fibrinogen, and prothrombin (Ref.2).

Sequence similarities: Belongs to the peptidase S1 family. Snake venom subfamily.Contains 1 peptidase S1 domain.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.