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Carotenoid Cleavage Dioxygenase 8, Chloroplastic (CCD8) Arabidopsis Thaliana Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1356498

MBS1356498 is a recombinant protein targeting CCD8. Also known as Recombinant Arabidopsis thaliana Carotenoid cleavage dioxygenase 8, chloroplastic (CCD8) , partial. Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Carotenoid cleavage dioxygenase 8, chloroplastic (CCD8), Recombinant Protein
Full Product Name
Recombinant Arabidopsis thaliana Carotenoid cleavage dioxygenase 8, chloroplastic (CCD8) , partial
Product Gene Name
CCD8 recombinant protein
Product Synonym Gene Name
CCD8
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
Molecular Weight
63,957 Da
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for Q8VY26
Species
Arabidopsis thaliana (Mouse-ear cress)
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of CCD8 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CCD8. It may not necessarily be applicable to this product.

NCBI GI #
30689501
NCBI GeneID
829417
NCBI Accession #
NP_195007.2
UniProt Primary Accession #
Q8VY26
UniProt Secondary Accession #
Q9M079
UniProt Related Accession #
Q8VY26
GenBank Nucleotide #
NM_119434.4
NCBI Official Full Name
carotenoid cleavage dioxygenase 8
NCBI Official Symbol
CCD8
NCBI Official Synonym Symbols
D8; carotenoid cleavage dioxygenase 8; MAX4; MORE AXILLARY BRANCHING 4; T16I18.20; T16I18_20
UniProt Protein Name
Carotenoid cleavage dioxygenase 8, chloroplastic
UniProt Synonym Protein Names
AtNCED8; Protein MORE AXILLARY BRANCHING 4; Protein MORE AXILLARY GROWTH 4
Protein Family
Carotenoid cleavage dioxygenase
UniProt Gene Name
CCD8
UniProt Synonym Gene Names
MAX4; NCED8
NCBI Protein Information
carotenoid cleavage dioxygenase 8

NCBI Summary for CCD8

Encodes a protein with similarity to carotenoid cleaving deoxygenases, the enzymes that cleave beta-carotene. Involved in the production of a graft transmissable signal to suppress axillary branching. Protein is localized to chloroplast stroma and expressed primarily in root tip. Mutants in the gene exhibit increased shoot branching, and light-dependent defects in hook opening and hypocotyl/root elongation. Only upregulated by auxin in the root and hypocotyl, and this is not required for the inhibition of shoot branching.
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UniProt Comments for CCD8

Involved in strigolactones biosynthesis by cleaving the C(27) 9-cis-10'-apo-beta-carotenal produced by CCD7. Produces the C(19) carlactone and a C8 hydroxyaldehyde. Also shows lower activity with all-trans-10'-apo-beta-carotenal producing a C9 dialdehyde and the C(18) 13-apo-beta-carotenone. Strigolactones are hormones that inhibit tillering and shoot branching through the MAX-dependent pathway, contribute to the regulation of shoot architectural response to phosphate-limiting conditions and function as rhizosphere signal that stimulates hyphal branching of arbuscular mycorrhizal fungi and trigger seed germination of root parasitic weeds. Also active on other carotenoid substrates like licopene or zeaxanthin.
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Research Articles on CCD8

1. The strigolactone biosynthesis genes MAX3 and MAX4 were drastically induced during dark incubation and treatment with the senescence-promoting phytohormone ethylene, suggesting that strigolactone is synthesized in the leaf during leaf senescence.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with CCD8 recombinant protein

 Products by Pathway  Pathway Diagram
 5-deoxystrigol Biosynthesis Pathway antibodies  5-deoxystrigol Biosynthesis Pathway Diagram
 5-deoxystrigol Biosynthesis Pathway antibodies  5-deoxystrigol Biosynthesis Pathway Diagram
 Carotenoid Biosynthesis Pathway antibodies  Carotenoid Biosynthesis Pathway Diagram
 Carotenoid Biosynthesis Pathway antibodies  Carotenoid Biosynthesis Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Diseases Associated With Visual Transduction Pathway antibodies  Diseases Associated With Visual Transduction Pathway Diagram
 Retinoid Metabolism And Transport Pathway antibodies  Retinoid Metabolism And Transport Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 The Canonical Retinoid Cycle In Rods (twilight Vision) Pathway antibodies  The Canonical Retinoid Cycle In Rods (twilight Vision) Pathway Diagram
 Visual Phototransduction Pathway antibodies  Visual Phototransduction Pathway Diagram
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