Active PAI-1 Functional Assay (SERPINE1) ELISA Kit for Dog

Background: This dog PAI-1 activity assay is intended for the quantitative determination of active plasminogen activator inhibitor type 1 (PAI-1) in dog plasma. For research use only.

Principle of the Assay: Functionally active PAI-1 present in plasma reacts with urokinase coated and dried on a microtiter plate. Latent or complexed PAI-1 will not bind to the plate and will not be detected. After appropriate washing steps, primary antibody binds to the captured protein. Excess antibody is washed away and bound polyclonal antibody is reacted with horseradish peroxidase conjugated secondary antibody. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of dog PAI-1. The amount of color development is directly proportional to the concentration of active PAI-1 in the sample.

Intended Uses: This dog PAI-1 activity assay is intended for the quantitative determination of active plasminogen activator inhibitor type 1 (PAI-1) in dog plasma. For research use only.

Small volumes of SERPINE1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS135937 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Active PAI-1 functional assay (SERPINE1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SERPINE1. The ELISA analytical biochemical technique of the MBS135937 kit is based on SERPINE1 antibody-SERPINE1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SERPINE1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SERPINE1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).