Immunoglobulin G (IgG) ELISA Kit for Hamster

This hamster IgG (Immunoglobulin G) ELISA kit measures IgG, the most abundant immunoglobulin in serum and a key player in the secondary immune response.

Background: IgG is the most abundant imunoglobulin in serum and is predominately involved in the secondary immune response. The IgG subclasses are designated 1, 2, 3 and 4 based on their relative prevalence in human serum.

Principle of the Assay: Armenian hamster IgG will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, horseradish peroxidase labeled polyclonal anti-Armenian hamster IgG primary antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of Armenian hamster IgG. Color development is directly proportional to the concentration of total IgG in the samples.

Intended Uses: The Armenian hamster imunoglobulin G (IgG) antigen assay is intended for the quantiative determination of total Armenian hamster IgG antigen in serum, plasma, hybridoma cell supernatants, ascites or other biological fluids

Small volumes of IgG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS136051 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Immunoglobulin G (IgG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IgG. The ELISA analytical biochemical technique of the MBS136051 kit is based on IgG antibody-IgG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IgG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IgG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).