Bone Morphogenetic 2 High-Sensitivity (BMP2) ELISA Kit for Bovine

This Bovine Bone Morphogenetic Protein 2 High-Sensitivity ELISA Kit is intended is a high-sensitivity sandwich ELISA kit for quantitative detection of Bovine BMP-2. 96wells/kit, with removable strips. Strip well format. Reagents for up to 96 tests.
This Bovine BMP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BMP-2 has been precoated onto 96-well plates. Standards (CHO, Q283-R396) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for BMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Bovine BMP-2 amount of sample captured in plate.
The capture antibody is monoclonal antibody from mouse, the detection antibody is monoclonal antibody from mouse. Expression system for standard: Bone morphogenetic protein-2 (BMP-2, BMP 2A) belongs to the transforming growth factor beta (TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice.1 BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase (MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis.2 Bone morphogenic proteins (BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion.3 The standard product used in this kit is recombinant Bovine BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.

Small volumes of BMP2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS136211 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Bone Morphogenetic 2 High-Sensitivity (BMP2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BMP2. The ELISA analytical biochemical technique of the MBS136211 kit is based on BMP2 antibody-BMP2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BMP2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BMP2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).