Brain Natriuretic (BNP-32) ELISA Kit for Canine

The Canine Brain Natriuretic Peptide (BNP-32) ELISA Kit is designed to detect a specific peptide and its related peptides based on the principle of "competitive" enzyme immunoassay. Strip well format. Reagents for up to 96 tests.
The immunoplate in this kit is pre-coated with secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate solution. The intensity of the yellow is directly proportional to the amount of biotinylated peptideSA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.

Small volumes of BNP-32 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS136350 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Brain Natriuretic Peptide (BNP-32) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BNP-32. The ELISA analytical biochemical technique of the MBS136350 kit is based on BNP-32 antibody-BNP-32 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BNP-32 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BNP-32. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).