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Cx3cl1/Fractalkine (CX3CL1) ELISA Kit for Canine

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS013787

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated CX3CL1 concentrations in Canine serum, plasma and other body fluids. Using Purified Canine CX3CL1 antibody to coat Microelisa Stripplate… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Cx3Cl1/Fractalkine, ELISA Kit
Full Product Name
Canine Cx3Cl1/Fractalkine ELISA Kit
Product Gene Name
CX3CL1 elisa kit
Species Reactivity
Canine
Assay Type
Sandwich
Samples
Serum, Plasma, Tissue Homogenate, Feces and Urine
Detection Range
0.5 ng/ml - 16 ng/ml.
Sensitivity
0.1 ng/ml.
Specificity
No significant cross-reactivity or interference between Canine CX3CL1 and analogues was observed.
Preparation and Storage
Store all reagents at 2-8 degree C
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Molecular Weight
42,187 Da
Chromosome Location
Chromosome: 16; NC_000016.9 (57406414..57418956). Location: 16q13
3D Structure
ModBase 3D Structure for Q6I9S9
Intended Uses
This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated CX3CL1 concentrations in Canine serum, plasma and other body fluids. Using Purified Canine CX3CL1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CX3CL1 and CX3CL1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CX3CL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Other Notes

Small volumes of CX3CL1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CX3CL1 purchase

MBS013787 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cx3Cl1/Fractalkine, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CX3CL1. The ELISA analytical biochemical technique of the MBS013787 kit is based on CX3CL1 antibody-CX3CL1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CX3CL1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CX3CL1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CX3CL1. It may not necessarily be applicable to this product.

NCBI GI #
48146969
NCBI GeneID
6376
NCBI Accession #
CAG33707.1
UniProt Primary Accession #
Q6I9S9
UniProt Related Accession #
P78423
NCBI Official Full Name
CX3CL1
NCBI Official Synonym Full Names
chemokine (C-X3-C motif) ligand 1
NCBI Official Symbol
CX3CL1
NCBI Official Synonym Symbols
NTN; NTT; CXC3; CXC3C; SCYD1; ABCD-3; C3Xkine; fractalkine; neurotactin
UniProt Protein Name
CX3CL1 protein
Protein Family
Fractalkine
UniProt Gene Name
CX3CL1
UniProt Entry Name
Q6I9S9_HUMAN
NCBI Protein Information
fractalkine; C-X3-C motif chemokine 1; small-inducible cytokine D1; CX3C membrane-anchored chemokine; small inducible cytokine subfamily D (Cys-X3-Cys), member-1; small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin)

UniProt Comments for CX3CL1

CX3CL1: The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1. Belongs to the intercrine delta family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16q13

Cellular Component: extracellular space; cell surface; extracellular region; plasma membrane; integral to membrane

Molecular Function: protein binding; chemokine activity; receptor binding

Biological Process: neutrophil chemotaxis; positive regulation of transforming growth factor-beta1 production; cytokine and chemokine mediated signaling pathway; leukocyte chemotaxis; defense response; angiogenesis involved in wound healing; chemotaxis; leukocyte adhesive activation; macrophage chemotaxis; positive regulation of angiogenesis; positive regulation of calcium-independent cell-cell adhesion; immune response; cell adhesion; lymphocyte chemotaxis; positive regulation of inflammatory response
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Research Articles on CX3CL1

1. The TLR3/IRF3/Fkn signaling pathway may, at least in part, mediate immune and inflammatory responses against viral infection in mesangial cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with CX3CL1 elisa kit

 Products by Pathway  Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Peptide Ligand-binding Receptors Pathway antibodies  Peptide Ligand-binding Receptors Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram

Organs/Tissues associated with CX3CL1 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >144 publications with CX3CL1 and Brain
 Lung Antibodies  >74 publications with CX3CL1 and Lung
 Kidney Antibodies  >71 publications with CX3CL1 and Kidney
 Muscle Antibodies  >71 publications with CX3CL1 and Muscle
 Liver Antibodies  >51 publications with CX3CL1 and Liver
 Heart Antibodies  >49 publications with CX3CL1 and Heart
 Nerve Antibodies  >39 publications with CX3CL1 and Nerve
 Skin Antibodies  >34 publications with CX3CL1 and Skin
 Intestine Antibodies  >29 publications with CX3CL1 and Intestine
 Eye Antibodies  >21 publications with CX3CL1 and Eye
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