Nuclear Inhibitor of Protein Phosphatase 1 (PPP1R8) Human Recombinant Protein

This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. [provided by RefSeq, Jul 2008]

NIPP-1: Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.4.-; Ribonuclease; Protein phosphatase, regulatory subunit; Motility/polarity/chemotaxis; Inhibitor

Chromosomal Location of Human Ortholog: 1p35.3

Cellular Component: nucleoplasm; spliceosome; cytoplasm; nuclear speck; nucleus

Molecular Function: protein binding; ribonuclease E activity; DNA binding; RNA binding; protein phosphatase type 1 regulator activity; endonuclease activity; type 1 serine/threonine specific protein phosphatase inhibitor activity

Biological Process: cell proliferation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; RNA splicing; RNA catabolic process; negative regulation of catalytic activity; mRNA processing