Complement Factor D (Adipsin) ELISA Kit for Human

The Human Complement Factor D ELISA Kit employs a quantitative enzyme immunoassay technique that measures Complement Factor D in Human samples. Strip well format. Reagents for up to 96 tests.
It is designed for detection of CFD in human plasma, serum, milk, saliva, CSF, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human CFD in less than 4 hours. A polyclonal antibody specific for human CFD has been pre-coated onto a 96-well microplate with removable strips. CFD in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human CFD, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

Small volumes of Complement Factor D (Adipsin) elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS139350 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Complement Factor D (Adipsin) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Complement Factor D (Adipsin). The ELISA analytical biochemical technique of the MBS139350 kit is based on Complement Factor D (Adipsin) antibody-Complement Factor D (Adipsin) antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Complement Factor D (Adipsin) antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Complement Factor D (Adipsin). Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).