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Splicing Factor 3b Subunit 4 (SF3B4) Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1398667

This gene encodes one of four subunits of the splicing factor 3B. This protein cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of… More details

$1165 / 0.02 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Splicing factor 3B subunit 4 (SF3B4), Recombinant Protein
Full Product Name
Recombinant Human Splicing factor 3B subunit 4 (SF3B4)
Product Gene Name
SF3B4 recombinant protein
Product Synonym Gene Name
SF3B4
Sequence
AAGPISERNQ DATVYVGGLD EKVSEPLLWE LFLQAGPVVN THMPKDRVTG QHQGYGFVEF LSEEDADYAI KIMNMIKLYG KPIRVNKASA HNKNLDVGAN IFIGNLDPEI DEKLLYDTFS AFGVILQTPK IMRDPDTGNS KGYAFINFAS FDASDAAIEA MNGQYLCNRP ITVSYAFKKD SKGERHGSAA ERLLAAQNPL SQADRPHQLF ADAPPPPSAP NPVVSSLGSG LPPPGMPPPG SFPPPVPPPG ALPPGIPPAM PPPPMPPGAA GHGPPSAGTP GAGHPGHGHS HPHPFPPGGM PHPGMSQMQL AHHGPHGLGH PHAGPPGSGG QPPPRPPPGM PHPGPPPMGM PPRGPPFGSP MGHPGPMPPH GMRGPPPLMP PHGYTGPPRP PPYGYQRGPL PPPRPTPRPP VPPRGPLRGP LPQ
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
Molecular Weight
44,386 Da
Sequence Positions
2-424. Full Length of Mature Protein
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
OMIM
154400
3D Structure
ModBase 3D Structure for Q15427
Species
Human
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of SF3B4 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SF3B4 purchase

MBS1398667 is a recombinant protein targeting SF3B4. Also known as Recombinant Human Splicing factor 3B subunit 4 (SF3B4). Host: E Coli or Yeast or Baculovirus or Mammalian Cell.

Additional Details

NCBI/Uniprot data below describe general gene information for SF3B4. It may not necessarily be applicable to this product.

NCBI GI #
5032069
NCBI GeneID
10262
NCBI Accession #
NP_005841.1
UniProt Primary Accession #
Q15427
UniProt Secondary Accession #
Q5SZ63
UniProt Related Accession #
Q15427
GenBank Nucleotide #
NM_005850.4
NCBI Official Full Name
splicing factor 3B subunit 4
NCBI Official Synonym Full Names
splicing factor 3b subunit 4
NCBI Official Symbol
SF3B4
NCBI Official Synonym Symbols
AFD1; Hsh49; SAP49; SF3b49
UniProt Protein Name
Splicing factor 3B subunit 4
UniProt Synonym Protein Names
Pre-mRNA-splicing factor SF3b 49 kDa subunit; Spliceosome-associated protein 49; SAP 49
Protein Family
Splicing factor
UniProt Gene Name
SF3B4
UniProt Synonym Gene Names
SAP49; SAP 49
NCBI Protein Information
splicing factor 3B subunit 4

NCBI Summary for SF3B4

This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA. [provided by RefSeq, Jul 2008]
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UniProt Comments for SF3B4

Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex (PubMed:27720643). SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed:12234937). May also be involved in the assembly of the 'E' complex. SF3B4 has been found in complex 'B' and 'C' as well (PubMed:10882114). Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron (PubMed:15146077).
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Research Articles on SF3B4

1. We identified two heterozygous frameshift mutations in the SF3B4 gene in three of the four fetuses investigated. The observed mutation was apparently de novo in one fetus for whom parental DNA was available. Thus, Acrofacial dysostosis syndrome of Rodriguez is an autosomal dominant condition and the recurrences identified in the initial report were likely due to gonadal mosaicism.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with SF3B4 recombinant protein

 Reference Product  PubMed Publications
 SF3B2 recombinant protein  >8 publications with SF3B4 and SF3B2
 BMPR1A recombinant protein  >2 publications with SF3B4 and BMPR1A

Pathways associated with SF3B4 recombinant protein

 Products by Pathway  Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies  Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
 Spliceosome Pathway antibodies  Spliceosome Pathway Diagram
 Spliceosome Pathway antibodies  Spliceosome Pathway Diagram
 Spliceosome, U2-snRNP Pathway antibodies  Spliceosome, U2-snRNP Pathway Diagram
 MRNA Splicing Pathway antibodies  MRNA Splicing Pathway Diagram
 MRNA Splicing - Major Pathway antibodies  MRNA Splicing - Major Pathway Diagram
 MRNA Splicing - Minor Pathway antibodies  MRNA Splicing - Minor Pathway Diagram
 MRNA Processing Pathway antibodies  MRNA Processing Pathway Diagram

Diseases associated with SF3B4 recombinant protein

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1 publications with SF3B4 and Neoplasms
 Neoplasms, Experimental Antibodies  >1 publications with SF3B4 and Neoplasms, Experimental
 Congenital Abnormalities Antibodies  >1 publications with SF3B4 and Congenital Abnormalities

Organs/Tissues associated with SF3B4 recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >2 publications with SF3B4 and Bone
 Heart Antibodies  >1 publications with SF3B4 and Heart
 Blood Antibodies  >1 publications with SF3B4 and Blood
 Embryonic Tissue Antibodies  >1 publications with SF3B4 and Embryonic Tissue
 Brain Antibodies  >1 publications with SF3B4 and Brain
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