Cysteine-Rich with EGF-Like Domain 2 (CRELD2) ELISA Kit for Human

This Human Cysteine-Rich With EGF-Like Domain Protein 2 (CRELD2) ELISA Kit is intended for quantitative detection of human CRELD2 in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.
This human CRELD2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CRELD2 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: A25-L353) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CRELD2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substmousee TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.
The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. Expression system for standard: Cysteine-rich with EGF-like domain protein 2 is a protein that in humans is encoded by the CRELD2 gene. It is mapped to 22q13.33. CRELD2 was identified by searching a sequence database using CRELD1 as the query. Like CRELD1, with which it shares 38% amino acid identity, the deduced 321-amino acid CRELD2 protein contains EGF-like repeats and a highly conserved domain rich in glutamic acid and tryptophan (WE domain). The major distinction between CRELD1 and CRELD2 is that CRELD1 has 2 type III transmembrane domains in its C terminus, while CRELD2 has none. CRELD2 shares 67% and 69% amino acid identity with its Chinese hamster and mouse homologs, respectively.

Small volumes of CRELD2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS139899 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cysteine-Rich With EGF-Like Domain 2 (CRELD2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CRELD2. The ELISA analytical biochemical technique of the MBS139899 kit is based on CRELD2 antibody-CRELD2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CRELD2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CRELD2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).