Mouse Anti-HK3 Monoclonal Antibody – Cat# MBS140266

Hexokinase-3 (HK3) Antibody – Mouse Monoclonal

Name: HK3 Antibody; Monoclonal; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS140266
Price: 255 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS140266

Introduction: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. HK3 encodes hexokinase 3. Similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product… More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Hexokinase-3 (HK3), Monoclonal Antibody

Full Product Name

Mouse Anti Human Hexokinase-3

Product Gene Name

anti-HK3 antibody

Product Synonym Names

HK3 Antibody; Hexokinase-3, Mouse Anti Human; Hexokinase-3; EC 2.7.1.1; Hexokinase type III; HK III; HXK3; HK3

Clonality

Monoclonal

Isotype

IgG1 heavy chain and kappa light chain

Clone Number

P4F5AT

Host

Mouse

Form/Format

1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.

Purity/Purification

Hexokinase-3 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.

Preparation and Storage

For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.
12 months at -20 degree C. 1 month at 4 degree C.

Molecular Weight

99,025 Da

OMIM

142570

Immunogen

Anti-human Hexokinase-3 mAb is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human Hexokinase-3 amino acids 1-923 purified from E Coli.

Product Categories/Family for anti-HK3 antibody

Monoclonal Antibodies; Anti-Human Enzymes; Monoclonal Antibodies; Anti Human Enzyme

Applications Tested/Suitable for anti-HK3 antibody

ELISA (EIA), Western Blot (WB)

Application Notes for anti-HK3 antibody

Hexokinase-3 antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000.

Other Notes

Small volumes of anti-HK3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HK3 purchase

MBS140266 is an antibody targeting HK3. Also known as Mouse Anti Human Hexokinase-3. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for HK3. It may not necessarily be applicable to this product.

NCBI GI #

194097330

NCBI GeneID

3101

NCBI Accession #

NP_002106.2

UniProt Secondary Accession #

Q8N1E7

UniProt Related Accession #

P52790

GenBank Nucleotide #

NM_002115.2

NCBI Official Full Name

hexokinase-3

NCBI Official Synonym Full Names

hexokinase 3 (white cell)

NCBI Official Symbol

HK3

NCBI Official Synonym Symbols

HXK3; HKIII

UniProt Protein Name

Hexokinase-3

UniProt Synonym Protein Names

Hexokinase type III; HK III

Protein Family

Probable histidine kinase

UniProt Gene Name

HK3

UniProt Synonym Gene Names

HK III

UniProt Entry Name

HXK3_HUMAN

NCBI Protein Information

hexokinase-3; ATP:D-hexose 6-phosphotransferase; HK III; hexokinase type III

NCBI Summary for HK3

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 3. Similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate. [provided by RefSeq, Apr 2009]

UniProt Comments for HK3

HK3: a glycolytic enzyme that catalyzes the reaction ATP + D-hexose = ADP + D-hexose 6-phosphate. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. An allosteric enzyme inhibited by its product glucose-6-phosphate (Glc-6-P). Acts as a ""glucose sensor"" by trapping glucose inside the cell by catalyzing its phosphorylation to produce Glc-6-P. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).

Protein type: Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - starch and sucrose; Kinase, other; Carbohydrate Metabolism - galactose; EC 2.7.1.1; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Enzyme, cellular metabolism; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Transferase

Chromosomal Location of Human Ortholog: 5q35.2

Cellular Component: protein complex; mitochondrion; cytosol

Molecular Function: hexokinase activity; enzyme binding; mannokinase activity; hormone binding; fructokinase activity; glucokinase activity; ATP binding

Biological Process: hexose metabolic process; glycolysis; hexose transport; carbohydrate metabolic process; glucose 6-phosphate metabolic process; carbohydrate phosphorylation; cell glucose homeostasis; pathogenesis; glucose transport; transmembrane transport

Research Articles on HK3

1. Altogether, our data provide an explanation for low HK3 and KLF5 expression in particular AML subtype and establish these genes as novel CEBPA targets during neutrophil differentiation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HK3 antibody

Reference Product	PubMed Publications
PFKFB2 antibody	>1 publications with HK3 and PFKFB2

Pathways associated with anti-HK3 antibody

Products by Pathway	Pathway Diagram
Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies	Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies	Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
Butirosin And Neomycin Biosynthesis Pathway antibodies	Butirosin And Neomycin Biosynthesis Pathway Diagram
Butirosin And Neomycin Biosynthesis Pathway antibodies	Butirosin And Neomycin Biosynthesis Pathway Diagram
Carbohydrate Digestion And Absorption Pathway antibodies	Carbohydrate Digestion And Absorption Pathway Diagram
Carbohydrate Digestion And Absorption Pathway antibodies	Carbohydrate Digestion And Absorption Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
Central Carbon Metabolism In Cancer Pathway antibodies	Central Carbon Metabolism In Cancer Pathway Diagram
Central Carbon Metabolism In Cancer Pathway antibodies	Central Carbon Metabolism In Cancer Pathway Diagram

Diseases associated with anti-HK3 antibody

Disease Name	Pubmed Publications
Carcinoma Antibodies	>10 publications with HK3 and Carcinoma
Hyperplasia Antibodies	>7 publications with HK3 and Hyperplasia
Skin Neoplasms Antibodies	>4 publications with HK3 and Skin Neoplasms
Liver Neoplasms Antibodies	>2 publications with HK3 and Liver Neoplasms
Carcinoma, Hepatocellular Antibodies	>2 publications with HK3 and Carcinoma, Hepatocellular
Leukemia, Promyelocytic, Acute Antibodies	>2 publications with HK3 and Leukemia, Promyelocytic, Acute
Liver Diseases Antibodies	>2 publications with HK3 and Liver Diseases
Neoplasms, Experimental Antibodies	>1 publications with HK3 and Neoplasms, Experimental
Carcinoma, Squamous Cell Antibodies	>1 publications with HK3 and Carcinoma, Squamous Cell
Cell Transformation, Neoplastic Antibodies	>1 publications with HK3 and Cell Transformation, Neoplastic

Organs/Tissues associated with anti-HK3 antibody

Organ/Tissue Name	Pubmed Publications
Prostate Antibodies	>54 publications with HK3 and Prostate
Blood Antibodies	>24 publications with HK3 and Blood
Brain Antibodies	>5 publications with HK3 and Brain
Skin Antibodies	>5 publications with HK3 and Skin
Testis Antibodies	>5 publications with HK3 and Testis
Lung Antibodies	>3 publications with HK3 and Lung
Muscle Antibodies	>2 publications with HK3 and Muscle
Connective Tissue Antibodies	>1 publications with HK3 and Connective Tissue