Mouse Anti-PPP1R14A Monoclonal Antibody – Cat# MBS140305

Phosphatase-1 (PPP1R14A) Antibody – Mouse Monoclonal

Name: PPP1R14A Antibody; Monoclonal; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS140305
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS140305

Introduction: PPP1R14A is a phosphorylation-dependent inhibitor of smooth muscle myosin phosphatase. Inhibition of PPP1R14A results to increased myosin phosphorylation and enhances smooth muscle contraction in the absence of increased intracellular Ca(2+)… More details

$250 / 0.005 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Phosphatase-1 (PPP1R14A), Monoclonal Antibody

Full Product Name

Mouse Anti Human Protein Phosphatase-1 Regulatory Subunit-14A

Product Gene Name

anti-PPP1R14A antibody

Product Synonym Names

PPP1R14A Antibody; Protein Phosphatase-1 Regulatory Subunit-14A, Mouse Anti Human; Protein phosphatase 1 regulatory subunit 14A; 17 kDa PKC-potentiated inhibitory protein of PP1; CPI17; CPI-17; PPP1INL; PPP1R14A

Clonality

Monoclonal

Isotype

IgG2a heavy chain and kappa light chain

Clone Number

P4H10AT

Host

Mouse

Form/Format

1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.

Purity/Purification

PPP1R14A antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.

Preparation and Storage

For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.
12 months at -20 degree C. 1 month at 4 degree C.

Molecular Weight

13,480 Da

OMIM

608153

Immunogen

Anti-human PPP1R14A mAb is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with recombinant human PPP1R14A amino acids 1-147 purified from E Coli.

Product Categories/Family for anti-PPP1R14A antibody

Monoclonal Antibodies; Anti-Human Enzymes; Monoclonal Antibodies; Anti Human Enzyme

Applications Tested/Suitable for anti-PPP1R14A antibody

ELISA (EIA), Western Blot (WB)

Application Notes for anti-PPP1R14A antibody

PPP1R14A antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000. Recommended starting dilution is 1:500.

Other Notes

Small volumes of anti-PPP1R14A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PPP1R14A purchase

MBS140305 is an antibody targeting PPP1R14A. Also known as Mouse Anti Human Protein Phosphatase-1 Regulatory Subunit-14A. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PPP1R14A. It may not necessarily be applicable to this product.

NCBI GI #

345441807

NCBI GeneID

94274

NCBI Accession #

NP_001230876.1

UniProt Secondary Accession #

Q7Z4X7; Q96S54

UniProt Related Accession #

Q96A00

GenBank Nucleotide #

NM_001243947.1

NCBI Official Full Name

protein phosphatase 1 regulatory subunit 14A isoform 2

NCBI Official Synonym Full Names

protein phosphatase 1, regulatory (inhibitor) subunit 14A

NCBI Official Symbol

PPP1R14A

NCBI Official Synonym Symbols

CPI17; CPI-17; PPP1INL

UniProt Protein Name

Protein phosphatase 1 regulatory subunit 14A

UniProt Synonym Protein Names

17 kDa PKC-potentiated inhibitory protein of PP1; Protein kinase C-potentiated inhibitor protein of 17 kDa; CPI-17

Protein Family

Protein phosphatase 1 regulatory

UniProt Gene Name

PPP1R14A

UniProt Synonym Gene Names

CPI17; PPP1INL; CPI-17

UniProt Entry Name

PP14A_HUMAN

NCBI Protein Information

protein phosphatase 1 regulatory subunit 14A; 17 kDa PKC-potentiated inhibitory protein of PP1; 17-KDa protein; 17-kDa PKC-potentiated inhibitory protein of PP1; protein kinase C-potentiated inhibitor protein of 17 kDa

NCBI Summary for PPP1R14A

The protein encoded by this gene belongs to the protein phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Sep 2011]

UniProt Comments for PPP1R14A

PPP1R14A: a phosphorylation-dependent inhibitory subunit of protein phosphatase 1 (PP-1A). Phosphorylation by PKC regulates its binding to PP-1A. Acts co-operatively to inhibit myosin light chain phosphatase (MLCP) activity. Inhibition leads to increased myosin phosphorylation and enhances smooth muscle contraction in the absence of increased intracellular Ca(2 ) concentration. Involved in the regulation of cell morphology, re-organisation of microfilaments, and cell spreading. Also expressed in human platelets and in brain where it may play a role in cerebellar long-term synaptic depression. Phosphorylation by ILK or Rho kinase enhances its ability to inhibit PP-1A.

Protein type: Inhibitor; Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: cytoplasm

Molecular Function: protein phosphatase inhibitor activity

Biological Process: regulation of phosphorylation; negative regulation of catalytic activity

Research Articles on PPP1R14A

1. CPI-17 has a role in restoring skin homoeostasis in cutaneous field of cancerization lesions

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PPP1R14A antibody

Reference Product	PubMed Publications
PPP1R12A antibody	>30 publications with PPP1R14A and PPP1R12A
PRKCA antibody	>2 publications with PPP1R14A and PRKCA
ILK antibody	>2 publications with PPP1R14A and ILK
PPP1R12B antibody	>1 publications with PPP1R14A and PPP1R12B
PPP1CB antibody	>1 publications with PPP1R14A and PPP1CB

Pathways associated with anti-PPP1R14A antibody

Products by Pathway	Pathway Diagram
Vascular Smooth Muscle Contraction Pathway antibodies	Vascular Smooth Muscle Contraction Pathway Diagram
Vascular Smooth Muscle Contraction Pathway antibodies	Vascular Smooth Muscle Contraction Pathway Diagram

Diseases associated with anti-PPP1R14A antibody

Disease Name	Pubmed Publications
Disease Models, Animal Antibodies	>14 publications with PPP1R14A and Disease Models, Animal
Inflammation Antibodies	>12 publications with PPP1R14A and Inflammation
Hypertension Antibodies	>9 publications with PPP1R14A and Hypertension
Necrosis Antibodies	>9 publications with PPP1R14A and Necrosis
Hyperplasia Antibodies	>4 publications with PPP1R14A and Hyperplasia
Hypertrophy Antibodies	>4 publications with PPP1R14A and Hypertrophy
Liver Diseases Antibodies	>3 publications with PPP1R14A and Liver Diseases
Fibrosis Antibodies	>1 publications with PPP1R14A and Fibrosis
Weight Gain Antibodies	>1 publications with PPP1R14A and Weight Gain
Liver Cirrhosis Antibodies	>1 publications with PPP1R14A and Liver Cirrhosis

Organs/Tissues associated with anti-PPP1R14A antibody

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>18 publications with PPP1R14A and Lung
Heart Antibodies	>12 publications with PPP1R14A and Heart
Intestine Antibodies	>11 publications with PPP1R14A and Intestine
Brain Antibodies	>6 publications with PPP1R14A and Brain
Stomach Antibodies	>5 publications with PPP1R14A and Stomach
Uterus Antibodies	>3 publications with PPP1R14A and Uterus
Eye Antibodies	>3 publications with PPP1R14A and Eye
Embryonic Tissue Antibodies	>3 publications with PPP1R14A and Embryonic Tissue
Prostate Antibodies	>2 publications with PPP1R14A and Prostate
Testis Antibodies	>2 publications with PPP1R14A and Testis