Mouse Anti-PFKM Monoclonal Antibody – Cat# MBS140461

Phosphofructokinase (PFKM) Antibody – Mouse Monoclonal

Name: PFKM Antibody; Monoclonal; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS140461
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS140461

Introduction: PFKM is a regulatory glycolytic enzyme that converts fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. Three phosphofructokinase isozymes exist in humans: muscle,… More details

$250 / 0.005 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Phosphofructokinase (PFKM), Monoclonal Antibody

Full Product Name

Mouse Anti Human Phosphofructokinase Muscle

Product Gene Name

anti-PFKM antibody

Product Synonym Names

PFKM Antibody; Phosphofructokinase Muscle, Mouse Anti Human; EC 2.7.1.11; GSD7; PFK-1; PFK1; PFKA; PFKX; Phosphofructokinase-M; Phosphofructokinase 1; Phosphohexokinase; Phosphofructo-1-kinase isozyme A; MGC8699; PFKM

Clonality

Monoclonal

Isotype

IgG2a heavy chain and Kappa light chain

Clone Number

PAT2F11A

Host

Mouse

Form/Format

1mg/ml containing PBS, pH-7.4, 10% Glycerol and 0.01% Sodium Azide.
Sterile Filtered colorless solution.

Purity/Purification

PFKM antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.

Preparation and Storage

For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.
12 months at -20 degree C. 1 month at 4 degree C.

Molecular Weight

37,678 Da

Immunogen

Anti-human PFKM mAb, clone PAT2F11A, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PFKM protein.

Product Categories/Family for anti-PFKM antibody

Monoclonal Antibodies; Anti-Human Enzymes; Monoclonal Antibodies; Anti Human Enzyme

Applications Tested/Suitable for anti-PFKM antibody

ELISA (EIA), Western Blot (WB)

Application Notes for anti-PFKM antibody

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:2000. Recommended starting dilution is 1:2000.

Other Notes

Small volumes of anti-PFKM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PFKM purchase

MBS140461 is an antibody targeting PFKM. Also known as Mouse Anti Human Phosphofructokinase Muscle. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PFKM. It may not necessarily be applicable to this product.

NCBI GI #

627777927

NCBI Accession #

AHY47148.1

NCBI Official Full Name

phosphofructokinase

UniProt Protein Name

Phosphofructokinase

UniProt Synonym Protein Names

Phosphofructokinase

Protein Family

ATP-dependent 6-phosphofructokinase

UniProt Gene Name

RradSPS_1865

UniProt Entry Name

A0A023X490_9ACTN

UniProt Comments for PFKM

Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis. Consistently, PPi-PFK can replace the enzymes of both the forward (ATP-PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.