Neuroepithelial Cell-Transforming Gene 1 (NET1) Human Recombinant Protein

Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.

Isolation of a novel oncogene, NET1, from neuroepithelioma cells by expression cDNA cloning.Chan A.M.-L., Takai S., Yamada K., Miki T.Oncogene 12:1259-1266(1996) The activity of guanine exchange factor NET1 is essential for transforming growth factor-beta-mediated stress fiber formation.Shen X., Li J., Hu P.P.-C., Waddell D., Zhang J., Wang X.-F.J. Biol. Chem. 276:15362-15368(2001) A quantitative atlas of mitotic phosphorylation.Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.Sci. Signal. 2:RA46-RA46(2009) The nuclear guanine nucleotide exchange factors Ect2 and Net1 regulate RhoB-mediated cell death after DNA damage.Srougi M.C., Burridge K.PLoS ONE 6:E17108-E17108(2011) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) Crystal structure of the RhoGEF domain of human neuroepithelial cell-transforming gene 1 protein.Structural genomics consortium (SGC) Submitted (FEB-2009) to the PDB data bankThe consensus coding sequences of human breast and colorectal cancers.Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V., Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.Science 314:268-274(2006)

Small volumes of NET1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS1406386 is a recombinant protein targeting NET1. Also known as Recombinant Human Neuroepithelial cell-transforming gene 1 protein. Host: E Coli or Yeast or Baculovirus or Mammalian Cell.