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Glutamic-Pyruvate Transaminase (GPT) Rat Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS140776

GPT Rat Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 519 amino acids (1-496 a.a) and having a molecular mass of 57.5kDa. GPT is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary… More details

$250 / 0.005 mg +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Glutamic-Pyruvate Transaminase (GPT), Active Protein
Full Product Name
Recombinant Rat Glutamic-Pyruvate Transaminase
Product Gene Name
Product Synonym Gene Name
rGPT
Product Synonym Names
GPT Rat; Glutamic-Pyruvate Transaminase Rat Recombinant; Alanine aminotransferase 1 (EC:2.6.1.2); ALT1; Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1; Gpt; Aat1; Gpt1
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAS RVND QSQASRNGLK GKVLTLDTMN PCVRRVEYAV RGPIVQRALE LEQELRQGVK KPFTEVIRAN IGDAQAMGQR PITFFRQVLA LCVYPNLLSS PDFPEDAKRR AERILQACGG HSLGAYSISS GIQPIREDVA QYIERRDGGI PADPNNIFLS TGASDAIVTM LKLLVSGEGR ARTGVLIPIP QYPLYSAALA ELDAVQVDYY LDEERAWALD IAELRRALCQ ARDRCCPRVL CVINPGNPTG QVQTRECIEA VIRFAFKEGL FLMADEVYQD NVYAEGSQFH SFKKVLMEMG PPYSTQQELA SFHSVSKGYM GECGFRGGYV EVVNMDAEVQ KQMGKLMSVR LCPPVPGQAL MDMVVSPPTP SEPSFKQFQA ERQEVLAELA AKAKLTEQVF NEAPGIRCNP VQGAMYSFPQ VQLPLKAVQR AQELGLAPDM FFCLCLLEET GICVVPGSGF GQQEGTYHFR MTILPPMEKL RLLLEKLSHF HAKFTHEYS.
Host
E Coli
Form/Format
GPT protein solution (1mg/ml) containing Phosphate Buffered Saline pH 7.4 and 10% glycerol.
Sterile Filtered colorless solution.
Purity/Purification
Greater than 90.0% as determined by SDS-PAGE.
Molecular Weight
55,110 Da
3D Structure
ModBase 3D Structure for P25409
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Biological Activity
Specific activity is > 60units/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37C.

Product Categories/Family for GPT active protein

Other Notes

Small volumes of GPT active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GPT purchase

MBS140776 is an active protein targeting GPT. Also known as Recombinant Rat Glutamic-Pyruvate Transaminase. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for GPT. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
alanine aminotransferase 1
NCBI Official Synonym Full Names
glutamic-pyruvate transaminase (alanine aminotransferase)
NCBI Official Symbol
Gpt
NCBI Official Synonym Symbols
ALAT; Gpt1
UniProt Protein Name
Alanine aminotransferase 1
UniProt Synonym Protein Names
Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1
UniProt Gene Name
Gpt
UniProt Synonym Gene Names
Aat1; Gpt1; ALT1; GPT 1
UniProt Entry Name
ALAT1_RAT
NCBI Protein Information
alanine aminotransferase 1

UniProt Comments for GPT

GPT: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; EC 2.6.1.2; Transferase

Cellular Component: extracellular space

Molecular Function: alanine transaminase activity
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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