Human RNGTT Recombinant Protein – Cat# MBS140783

RNA Guanylyltransferase and 5'-Phosphatase (RNGTT) Human Recombinant Protein

Name: RNGTT Recombinant Protein; Human; MyBioSource
Brand: MyBioSource
SKU: MBS140783
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS140783

Introduction: RNA Guanylyltransferase And 5'-Phosphatase also known as RNGTT is a bifunctional mRNA-capping enzyme which exhibits RNA 5'-triphosphatase activity in the N-terminal section and mRNA guanylyltransferase activity in the C-terminal section. In… More details

$250 / 0.002 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

RNA Guanylyltransferase And 5'-Phosphatase (RNGTT), Recombinant Protein

Full Product Name

Recombinant Human RNA Guanylyltransferase And 5'-Phosphatase

Product Gene Name

RNGTT recombinant protein

Product Synonym Names

RNGTT Human; RNA Guanylyltransferase And 5'-Phosphatase Human Recombinant; RNA Guanylyltransferase And 5'-Phosphatase; CAP1A; RNA Guanylyltransferase And 5-Phosphatase; HCAP1; HCE1; HCE; MRNA-Capping Enzyme; HCAP 3; mRNA-capping enzyme

Sequence

MGSSHHH HHH SSGLVPRGSH MGSMAH NKIP PRWLNCPRRG QPVAGRFLPL KTMLGPRYDS QVAEENRFHP SMLSNYLKSL KVKMGLLVDL TNTSRFYDRN DIEKEGIKYI KLQCKGHGEC PTTENTETFI RLCERFNERN PPELIGVHCT HGFNRTGFLI CAFLVEKMDW SIEAAVATFA QARPPGIYKG DYLKELFRRY GDIEEAPPPP LLPDWCFEDD EDEDEDEDGK KESEPGSSAS FGKRRKERLK LGAIFLEGVT VKGVTQVTTQ PKLGEVQQKC HQFCGWEGSG FPGAQPVSMD KQNIKLLDLK PYKVSWKADG TRYMMLIDGT NEVFMIDRDN SVFHVSNLEF PFRKDLRMHL SNTLLDGEMI IDRVNGQAVP RYLIYDIIKF NSQPVGDCDF NVRLQCIERE IISPRHEKMK TGLIDKTQEP FSVRNKPFFD ICTSRKLLEG NFAKEVSHEM DGLIFQPTGK YKPGRCDDIL KWKPPSLNSV DFRLKITRMG GEGLLPQNVG LLYVGGYERP FAQIKVTKEL KQYDNKIIEC KFENNSWVFM RQRTDKSFPN AYNTAMAVCN SISNPVTKEM LFEFIDRCTA ASQGQKRKHH LDPDTELMPP PPPKRPRPLT

Host

E Coli

Form/Format

RNGTT protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 7.5), 0.2M NaCl, 40% glycerol, 2mM DTT and 0.1mM PMSF.
Sterile filtered colorless solution.

Purity/Purification

Greater than 85.0% as determined by SDS-PAGE.

Preparation and Storage

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Molecular Weight

61,705 Da

OMIM

603512

3D Structure

ModBase 3D Structure for O60942

Product Categories/Family for RNGTT recombinant protein

Enzymes

Other Notes

Small volumes of RNGTT recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RNGTT purchase

MBS140783 is a recombinant protein targeting RNGTT. Also known as Recombinant Human RNA Guanylyltransferase And 5'-Phosphatase. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for RNGTT. It may not necessarily be applicable to this product.

NCBI GI #

556695469

NCBI GeneID

8732

NCBI Accession #

NP_001273355.1

UniProt Primary Accession #

O60942

UniProt Secondary Accession #

O43483; O60257; O60351; Q5TCW8; Q8WUM8; E1P513; E1P514

UniProt Related Accession #

O60942

GenBank Nucleotide #

NM_001286426.1

NCBI Official Full Name

mRNA-capping enzyme isoform b

NCBI Official Synonym Full Names

RNA guanylyltransferase and 5'-phosphatase

NCBI Official Symbol

RNGTT

NCBI Official Synonym Symbols

HCE; HCE1; hCAP; CAP1A

UniProt Protein Name

mRNA-capping enzyme

UniProt Synonym Protein Names

HCAP1; HCEIncluding the following 2 domains:Polynucleotide 5'-triphosphatase (EC:3.1.3.33)Alternative name(s):mRNA 5'-triphosphatase; TPase

UniProt Gene Name

RNGTT

UniProt Synonym Gene Names

CAP1A; TPase; GTase

UniProt Entry Name

MCE1_HUMAN

NCBI Protein Information

mRNA-capping enzyme

UniProt Comments for RNGTT

RNGTT: a bifunctional mRNA capping enzyme with RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus. Four alternatively spliced human isoforms have been described. Isoform 1 and isoform 4 are expressed in cerebrum, cerebellum, thyroid, lung, heart, liver, kidney, spleen, large intestine, testis, skin and muscle. Isoforms 2, 3 and 4 lack mRNA 5'-guanylyltransferase activity due to disruptions of the GTPase domain.

Protein type: EC 2.7.7.50; EC 3.1.3.33; Motility/polarity/chemotaxis; Phosphatase (non-protein); Transferase

Chromosomal Location of Human Ortholog: 6q16

Cellular Component: nucleoplasm; nucleus

Molecular Function: mRNA guanylyltransferase activity; polynucleotide 5'-phosphatase activity; RNA guanylyltransferase activity; triphosphatase activity

Biological Process: mRNA capping; RNA processing; transcription from RNA polymerase II promoter

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with RNGTT recombinant protein

Products by Pathway	Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
HIV Infection Pathway antibodies	HIV Infection Pathway Diagram
HIV Life Cycle Pathway antibodies	HIV Life Cycle Pathway Diagram
Infectious Disease Pathway antibodies	Infectious Disease Pathway Diagram
Late Phase Of HIV Life Cycle Pathway antibodies	Late Phase Of HIV Life Cycle Pathway Diagram
RNA Pol II CTD Phosphorylation And Interaction With CE Pathway antibodies	RNA Pol II CTD Phosphorylation And Interaction With CE Pathway Diagram
RNA Pol II CTD Phosphorylation And Interaction With CE Pathway antibodies	RNA Pol II CTD Phosphorylation And Interaction With CE Pathway Diagram
RNA Polymerase II Transcription Pathway antibodies	RNA Polymerase II Transcription Pathway Diagram
Transcription Of The HIV Genome Pathway antibodies	Transcription Of The HIV Genome Pathway Diagram

Organs/Tissues associated with RNGTT recombinant protein

Organ/Tissue Name	Pubmed Publications
Eye Antibodies	>1 publications with RNGTT and Eye