• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Granulocyte-Colony Stimulating Factor (GCSF) Rat Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS140804

Introduction: GCSF is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Three transcript variants encoding three different isoforms have been found for the GCSF gene.… More details

$250 / 0.002 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Granulocyte-Colony Stimulating Factor (GCSF), Active Protein
Full Product Name
Recombinant Rat Granulocyte-Colony Stimulating Factor
Product Gene Name
GCSF active protein
Product Synonym Gene Name
rGCSF
Product Synonym Names
GCSF Rat; Granulocyte-Colony Stimulating Factor Rat Recombinant; Granulocyte colony stimulating factor; Protein Csf3; Csf3
Sequence
KKIPLLTVSS LPPSLPLPRS FLLKSLEQVR KIQARNTELL EQLCATYKLC HPEELVLFGH SLGIPKASLS SCSSQALQQT KCLSQLHSGL FLYQGLLQAL AGISSELAPT LDMLHLDVDN FATTIWQQME SLGVAPTVQP TQSTMPIFTS AFQRRAGGVL VTSYLQSFLE TAHHALHHLP RPAQKHFPES LFISI
Host
E Coli
Form/Format
Lyophilized from a 0.2 um filtered solution in 5mM Sodium Citrate, pH 4.0.
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity/Purification
Greater than 97.0% as determined by:(a)Analysis by RP-HPLC.(b)Analysis by SDS-PAGE.
Preparation and Storage
Lyophilized G-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution GCSF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Molecular Weight
23,660 Da
3D Structure
ModBase 3D Structure for P97712
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Biological Activity
The ED50 determined by a cell proliferation assay using murine NFS-60 cells is less than 0.05ng/ml, corresponding to a specific activity of > 2.0x 107 IU/mg.
Solubility
It is recommended to reconstitute the lyophilized GCSF in sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.

Product Categories/Family for GCSF active protein

Cytokines and Growth Factors

Other Notes

Small volumes of GCSF active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GCSF purchase

MBS140804 is an active protein targeting GCSF. Also known as Recombinant Rat Granulocyte-Colony Stimulating Factor. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for GCSF. It may not necessarily be applicable to this product.

NCBI GI #
81908538
NCBI Accession #
P97712
UniProt Primary Accession #
P97712
NCBI Official Full Name
Granulocyte colony stimulating factor
UniProt Protein Name
Granulocyte colony stimulating factor
UniProt Synonym Protein Names
Granulocyte colony stimulating factor
Protein Family
Granulocyte colony-stimulating factor
UniProt Gene Name
Csf3
UniProt Entry Name
P97712_RAT

UniProt Comments for GCSF

G-CSF: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Cytokine; Secreted; Secreted, signal peptide

Cellular Component: extracellular space

Molecular Function: cytokine activity; enzyme binding; granulocyte colony-stimulating factor receptor binding; growth factor activity

Biological Process: positive regulation of actin filament polymerization; positive regulation of cell proliferation; positive regulation of myeloid cell differentiation; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein binding; positive regulation of protein kinase B signaling cascade; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter; response to ethanol
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000