DYKDDDDK Antibody – Mouse Monoclonal

A fusion tag called DYKDDDDK consists of eight amino acids (AspTyrLysAspAspAspAspLys) including an enterokinase-cleavage site. DYKDDDDK is specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody denoted as M1 binds the peptide in the presence of bivalent metal cations preferably Ca(+). Elution is effected by chelating agents. Another strategy is competitive elution with excess of free DYKDDDDK Peptide. The DYKDDDDK peptide purifies and detects recombinant fusion proteins. DYKDDDDK peptide is useful in Western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization. DYKDDDDK peptide is a hydrophilic tag which significantly improves the detection and purification of recombinant fusion proteins.

Small volumes of anti-DYKDDDDK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS141207 is an antibody targeting DYKDDDDK. Also known as Mouse Anti DYKDDDDK peptide Clone PAT3G6AT. Reactive in Human. Host: Mouse.