Arabidopsis Thaliana ORTH1 Recombinant Protein – Cat# MBS1413634

E3 Ubiquitin-Protein Ligase ORTHRUS 1 (ORTH1) Arabidopsis Thaliana Recombinant Protein

Name: ORTH1 Recombinant Protein; Arabidopsis Thaliana; MyBioSource
Brand: MyBioSource
SKU: MBS1413634
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1413634

MBS1413634 is a recombinant protein targeting ORTH1. Also known as Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase ORTHRUS 1 (ORTH1) , partial. Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

E3 ubiquitin-protein ligase ORTHRUS 1 (ORTH1), Recombinant Protein

Full Product Name

Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase ORTHRUS 1 (ORTH1) , partial

Product Gene Name

ORTH1 recombinant protein

Product Synonym Gene Name

ORTH1

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Form/Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Purity/Purification

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Preparation and Storage

Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Molecular Weight

68,299 Da

Tag Information

This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.

Sterility

Sterile filter available upon request.

Endotoxin

Low endotoxin available upon request.

3D Structure

ModBase 3D Structure for Q9FKA7

Species

Arabidopsis thaliana (Mouse-ear cress)

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of ORTH1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ORTH1. It may not necessarily be applicable to this product.

NCBI GI #

15241791

NCBI GeneID

833951

NCBI Accession #

NP_198771.1

UniProt Primary Accession #

Q9FKA7

UniProt Secondary Accession #

Q67XP0

UniProt Related Accession #

Q9FKA7

GenBank Nucleotide #

NM_123317.4

NCBI Official Full Name

Zinc finger (C3HC4-type RING finger) family protein

NCBI Official Symbol

VIM3

NCBI Official Synonym Symbols

MIJ24.3; MIJ24_3; ORTH1; ORTHRUS 1; VARIANT IN METHYLATION 3

UniProt Protein Name

E3 ubiquitin-protein ligase ORTHRUS 1

UniProt Synonym Protein Names

Protein VARIANT IN METHYLATION 3; RING-type E3 ubiquitin transferase ORTHRUS 1Curated

Protein Family

E3 ubiquitin-protein ligase

UniProt Gene Name

ORTH1

UniProt Synonym Gene Names

VIM3

NCBI Protein Information

Zinc finger (C3HC4-type RING finger) family protein

NCBI Summary for ORTH1

Encodes the VIM3/ORTH1 protein that is similar to VIM1. This protein has an N-terminal PHD domain and two RING domains surrounding an SRA domain. The protein has been shown to bind to methylated cytosines of CG, CNG and CNN motifs via its SRA domain but has a preference for the former. This protein functions as an E3 ubiquitin ligase in vitro with members of the UBC8 family E2s. Either of the two RING domains present in the protein can promote ubiquitylation in vitro, but, not the PHD domain. Over-expression of ORTH1/VIM3 leads to decreased levels of FWA methylation, increased levels of FWA transcripts, and delayed flowering. Cen180 repeats are also hypomethylated in plants overexpressing this protein.

UniProt Comments for ORTH1

E3 ubiquitin-protein ligase. Participates in CpG methylation-dependent transcriptional regulation and epigenetic transcriptional silencing. Mediates ubiquitination with the E2 ubiquitin-conjugating enzymes UBC11, UBC8 and UBC8 homologs (e.g. UBC10, UBC11, UBC28 and UBC29) but not with UBC27, UBC30, UBC32, UBC34 and UBC36. Promotes methylation-mediated gene silencing leading, for example, to early flowering. Can bind to CpG, CpNpG, and CpNpN DNA motifs, with a strong preference for methylated forms, and with highest affinity for CpG substrate.

Research Articles on ORTH1

1. VIM proteins function in transcriptional regulation via their roles in the MET1 DNA methylation pathway.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with ORTH1 recombinant protein

Organ/Tissue Name	Pubmed Publications
Flower Antibodies	>1 publications with ORTH1 and Flower