Argonaute 2 (AGO2) Human Recombinant Protein

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

AGO2: the endonuclease in RNA-induced silencing complexes (RISC) that cleaves siRNA/mRNA heteroduplexes bound to RISC. Ago2, along with Dicer and TRBP, are major components of RISC. Interacts with Dicer1 through its Piwi domain. Required for proper fibroblast growth factor signaling during gastrulation. Essential for embryonic development as well as RNA-mediated gene silencing (RNAi).

Protein type: RNA processing; RNA-binding; EC 3.1.26.n2

Chromosomal Location of Human Ortholog: 8q24

Cellular Component: nucleoplasm; polysome; mRNA cap complex; membrane; cytoplasm; ribonucleoprotein complex; cytosol

Molecular Function: mRNA binding; endoribonuclease activity; protein binding; miRNA binding; siRNA binding; translation initiation factor activity; metal ion binding; RNA 7-methylguanosine cap binding

Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of translational initiation; Notch signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; transcription, DNA-dependent; RNA interference, siRNA loading onto RISC; translation; miRNA-mediated gene silencing, mRNA cleavage; miRNA-mediated gene silencing, miRNA loading onto RISC; miRNA-mediated gene silencing, negative regulation of translation; post-embryonic development; regulation of transcription, DNA-dependent; pre-microRNA processing; translational initiation; innate immune response; RNA-mediated gene silencing; gene expression; posttranscriptional gene silencing