Human IL12 Active Protein – Cat# MBS141431

Interleukin 12 (IL12) Human Active Protein

Name: IL12 Active Protein; Interleukin 12; Human; MyBioSource
Brand: MyBioSource
SKU: MBS141431
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS141431

Introduction: Interleukin 12 subunit beta/alpha or IL12b/IL12a is a growth factor cytokine which increases the lytic activity of NK/lymphokine-activated killer cells, activated T and NK cells and prompt IFN-gamma production (through resting PBMC). IL12b/IL12a… More details

$250 / 0.002 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Interleukin 12 (IL12), Active Protein

Full Product Name

Recombinant Human Interleukin 12, CHO

Product Gene Name

IL12 active protein

Product Synonym Gene Name

CHO

Product Synonym Names

23-219aa (IL12A) / 23-328aa (IL12B); Interleukin 12 (subunit beta/alpha); IL12 His; NKSF1; NKSF; CTL maturation factor (TCMF); Cytotoxic lymphocyte maturation factor (CLMF); TSF; Edodekin-alpha; IL-12; IL12 Human, CHO; Interleukin-12 Human Recombinant, CHO

Sequence

Amino acid: IL-12 is a heterodime r of IL-12A and IL-12B linked through a disulfide- bond:
A RNLPVATP DPGMFPCLHH SQNLLRAVSN MLQKARQTLE FYPCTSEEID HEDITKDKTS TVEACLPLEL TKNESCLNSR ETSFITNGSC LASRKTSFMM ALCLSSIYED LKMYQVEFKT MNAKLLMDPK RQIFLDQNML AVIDELMQAL NFNSETVPQK SSLEEPDFYK TKIKLCILLH AFRIRAVTID RVMSYLNAS.
IL-12B :
IWELK KDV YVVELDWYPD APGEMVVLTC DTPEEDGITW TLDQSSEVLG SGKTLTIQVK EFGDAGQYTC HKGGEVLSHS LLLLHKKEDG IWSTDILKDQ KEPKNKTFLR CEAKNYSGRF TCWWLTTIST DLTFSVKSSR GSSDPQGVTC GAATLSAERV RGDNKEYEYS VECQEDSACP AAEESLPIEV MVDAVHKLKY ENYTSSFFIR DIIKPDPPKN LQLKPLKNSR QVEVSWEYPD TWSTPHSYFS LTFCVQVQGK SKREKKDRVF TDKTSATVIC RKNASISVRA QDRYYSSSWS EWASVPCS.

Host

Baculovirus

Form/Format

Sterile filtered white lyophilized (freeze-dried) powder.
Lyophilized from a 0.2um filtered concentrated solution in PBS, pH 7.2.

Purity/Purification

>97.0% as determined by analysis by SDS-PAGE.

Preparation and Storage

Lyophilized Interleukin 12 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C.
Upon reconstitution PDGF BB should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.
Please prevent freeze-thaw cycles.

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility

It is recommended to reconstitute the lyophilized Interleukin 12 in sterile 18Momega-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Biological Activity

Determined by cell proliferation assay using PHA-stimulated human T lymphoblasts. The ED50 for this effect is 0.01-0.05ng/mL corresponding to a specific activity of 1.1 × 104 units/?g, which is calibrated against rHuIL-12 WHO Standard.

Product Categories/Family for IL12 active protein

CYTOKINES AND GROWTH FACTORS

Other Notes

Small volumes of IL12 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL12 purchase

MBS141431 is an active protein targeting IL12. Also known as Recombinant Human Interleukin 12, CHO. Host: Baculovirus.

Additional Details

NCBI/Uniprot data below describe general gene information for IL12. It may not necessarily be applicable to this product.

NCBI GI #

4323579

NCBI GeneID

3592

UniProt Related Accession #

P29459

NCBI Official Full Name

interleukin 12

NCBI Official Synonym Full Names

interleukin 12A

NCBI Official Symbol

IL12A

NCBI Official Synonym Symbols

P35; CLMF; NFSK; NKSF1; IL-12A

Protein Family

Interleukin-12 receptor

NCBI Protein Information

interleukin-12 subunit alpha

NCBI Summary for IL12

This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]

Research Articles on IL12

1. The distributions of IFNgamma rs2430561, IFNgamma rs35314021, IL12A rs568408, IL12A rs2243115 and IL12B rs3212227 SNP did not differ between BCG osteitis patients and Finnish population-based controls.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with IL12 active protein

Reference Product	PubMed Publications
IL12B active protein	>56 publications with IL12 and IL12B
IL4 active protein	>21 publications with IL12 and IL4
IL12RB2 active protein	>19 publications with IL12 and IL12RB2
IFNG active protein	>16 publications with IL12 and IFNG
IL12RB1 active protein	>14 publications with IL12 and IL12RB1
IL1B active protein	>13 publications with IL12 and IL1B
STAT1 active protein	>8 publications with IL12 and STAT1
JAK2 active protein	>3 publications with IL12 and JAK2
TYK2 active protein	>3 publications with IL12 and TYK2
TBX21 active protein	>2 publications with IL12 and TBX21

Pathways associated with IL12 active protein

Products by Pathway	Pathway Diagram
African Trypanosomiasis Pathway antibodies	African Trypanosomiasis Pathway Diagram
African Trypanosomiasis Pathway antibodies	African Trypanosomiasis Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram

Diseases associated with IL12 active protein

Disease Name	Pubmed Publications
Inflammation Antibodies	>89 publications with IL12 and Inflammation
Necrosis Antibodies	>56 publications with IL12 and Necrosis
Disease Models, Animal Antibodies	>48 publications with IL12 and Disease Models, Animal
Brain Diseases Antibodies	>24 publications with IL12 and Brain Diseases
Liver Diseases Antibodies	>23 publications with IL12 and Liver Diseases
Multiple Sclerosis Antibodies	>21 publications with IL12 and Multiple Sclerosis
Hepatitis Antibodies	>15 publications with IL12 and Hepatitis
Neoplasms, Experimental Antibodies	>14 publications with IL12 and Neoplasms, Experimental
Liver Cirrhosis, Biliary Antibodies	>11 publications with IL12 and Liver Cirrhosis, Biliary
Fibrosis Antibodies	>8 publications with IL12 and Fibrosis

Organs/Tissues associated with IL12 active protein

Organ/Tissue Name	Pubmed Publications
Spleen Antibodies	>34 publications with IL12 and Spleen
Brain Antibodies	>19 publications with IL12 and Brain
Intestine Antibodies	>14 publications with IL12 and Intestine
Uterus Antibodies	>7 publications with IL12 and Uterus