Mouse Soluble msRANKL Active Protein – Cat# MBS141540

RANK Ligand (msRANKL) Mouse Soluble Active Protein

Name: msRANKL Active Protein; RANK Ligand; Mouse Soluble; MyBioSource
Brand: MyBioSource
SKU: MBS141540
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS141540

Soluble RANK Ligand or type II membrane protein, is part of the TNF proteins family. RANKL has been found to have an effect on the immune system and regulates bone regeneration and remodeling. This protein regulates cell apoptosis, bunds to OPG protein… More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

RANK Ligand (msRANKL), Active Protein

Full Product Name

Recombinant Mouse Soluble RANK Ligand (158-316 a.a), Active

Product Gene Name

msRANKL active protein

Product Synonym Names

msRANKL (158-316) Active; sRANKL Mouse (158-316) Active; Soluble RANK Ligand (158-316 a.a); Mouse Recombinant Active; Tumor necrosis factor ligand superfamily member 11; Osteoclast differentiation factor;ODF; Osteoprotegerin ligand; OPGL; Receptor activator of nuclear factor kappa-B ligand; RANKL; TNF-related activation-induced cytokine; TRANCE; CD254; Tnfsf11; Opgl; Rankl; Trance

Sequence

MKPEAQPFAH LTINAASIPS GSHKVTLSSW YHDRGWAKIS NMTLSNGKLR VNQDGFYYLY ANICFRHHET SGSVPTDYLQ LMVYVVKTSI KIPSSHNLMK GGSTKNWSGN SEFHFYSINV GGFFKLRAGE EISIQVSNPS LLDPDQDATY FGAFKVQDID

Host

E Coli

Form/Format

Sterile filtered colorless solution.
The sRANKL solution (1mg/ml) contains 0.1M NaCl and Tris-Hcl buffer (pH8.5).

Purity/Purification

Greater than 90.0% as determined by SDS-PAGE

Preparation and Storage

Store at 4 degree C if entire vial will be used within 2-4 weeks.
Store, frozen at -20 degree C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.

Molecular Weight

35,003 Da

3D Structure

ModBase 3D Structure for O35235

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Biological Activity

Determined by osteoclast differentiation assay using RAW 264.7 mouse monocyte/macrophage cells. ED50 range for this is <= 2ng/ml.

Product Categories/Family for msRANKL active protein

CYTOKINES AND GROWTH FACTORS

Other Notes

Small volumes of msRANKL active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for msRANKL purchase

MBS141540 is an active protein targeting msRANKL. Also known as Recombinant Mouse Soluble RANK Ligand (158-316 a.a), Active. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for msRANKL. It may not necessarily be applicable to this product.

NCBI GI #

114842415

NCBI GeneID

21943

NCBI Accession #

NP_035743.2

UniProt Primary Accession #

O35235

UniProt Secondary Accession #

O35306; Q3TWY5; Q9JJK8; Q9JJK9; Q9R1Y0

UniProt Related Accession #

O35235

GenBank Nucleotide #

NM_011613.3

NCBI Official Full Name

tumor necrosis factor ligand superfamily member 11

NCBI Official Synonym Full Names

tumor necrosis factor (ligand) superfamily, member 11

NCBI Official Symbol

Tnfsf11

NCBI Official Synonym Symbols

ODF; OPG; OPGL; RANKL; Ly109l; Trance

UniProt Protein Name

Tumor necrosis factor ligand superfamily member 11

UniProt Synonym Protein Names

Osteoclast differentiation factor; ODF; Osteoprotegerin ligand; OPGL; Receptor activator of nuclear factor kappa-B ligand; RANKL; TNF-related activation-induced cytokine

UniProt Gene Name

Tnfsf11

UniProt Synonym Gene Names

Opgl; Rankl; Trance; ODF; OPGL; RANKL; TRANCE

UniProt Entry Name

TNF11_MOUSE

NCBI Protein Information

tumor necrosis factor ligand superfamily member 11; OPG ligand; osteoprotegerin ligand; osteoclast differentiation factor; receptor activator of NF-kappaB ligand; TNF-related activation-induced cytokine; receptor activator of nuclear factor kappa B ligand

UniProt Comments for msRANKL

TNFSF11: Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Homotrimer. Up-regulated by T-cell receptor stimulation. Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid. Belongs to the tumor necrosis factor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: extracellular space; membrane; cytoplasm; extracellular region; integral to membrane; plasma membrane; intracellular

Molecular Function: protein binding; cytokine activity; tumor necrosis factor receptor superfamily binding; tumor necrosis factor receptor binding

Biological Process: ossification; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of osteoclast differentiation; multicellular organismal development; cytokine and chemokine mediated signaling pathway; positive regulation of JNK cascade; mammary gland epithelial cell proliferation; osteoclast differentiation; lymph node development; activation of JNK activity; positive regulation of corticotropin-releasing hormone secretion; activation of NF-kappaB transcription factor; positive regulation of homotypic cell-cell adhesion; calcium ion homeostasis; positive regulation of protein kinase B signaling cascade; monocyte chemotaxis; positive regulation of MAP kinase activity; organ morphogenesis; regulation of osteoclast differentiation; tumor necrosis factor-mediated signaling pathway; positive regulation of bone resorption; positive regulation of transcription from RNA polymerase II promoter; immune response; positive regulation of transcription factor activity; positive regulation of T cell activation; cell differentiation; positive regulation of phosphorylation; protein homooligomerization; bone resorption

Research Articles on msRANKL

1. The OPG/RANKL/RANK axis plays critical roles in controlling inflammation in ischemic brains.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with msRANKL active protein

Products by Pathway	Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
NF-kappa B Signaling Pathway antibodies	NF-kappa B Signaling Pathway Diagram
Osteoblast Pathway antibodies	Osteoblast Pathway Diagram
Osteoclast Pathway antibodies	Osteoclast Pathway Diagram
Osteoclast Differentiation Pathway antibodies	Osteoclast Differentiation Pathway Diagram
Osteoclast Differentiation Pathway antibodies	Osteoclast Differentiation Pathway Diagram
Prolactin Signaling Pathway antibodies	Prolactin Signaling Pathway Diagram
Prolactin Signaling Pathway antibodies	Prolactin Signaling Pathway Diagram
Rheumatoid Arthritis Pathway antibodies	Rheumatoid Arthritis Pathway Diagram

Diseases associated with msRANKL active protein

Disease Name	Pubmed Publications
Necrosis Antibodies	>1520 publications with msRANKL and Necrosis
Osteoporosis Antibodies	>1092 publications with msRANKL and Osteoporosis
Neoplasms Antibodies	>918 publications with msRANKL and Neoplasms
Inflammation Antibodies	>506 publications with msRANKL and Inflammation
Osteolysis Antibodies	>342 publications with msRANKL and Osteolysis
Cardiovascular Diseases Antibodies	>306 publications with msRANKL and Cardiovascular Diseases
Breast Neoplasms Antibodies	>226 publications with msRANKL and Breast Neoplasms
Alveolar Bone Loss Antibodies	>144 publications with msRANKL and Alveolar Bone Loss
Pain Antibodies	>124 publications with msRANKL and Pain
Osteopetrosis Antibodies	>121 publications with msRANKL and Osteopetrosis

Organs/Tissues associated with msRANKL active protein

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>4399 publications with msRANKL and Bone
Spleen Antibodies	>146 publications with msRANKL and Spleen
Thymus Antibodies	>39 publications with msRANKL and Thymus