Human SPP1 Active Protein – Cat# MBS141544

Osteopontin (SPP1) Human Active Protein

Name: SPP1 Active Protein; Osteopontin; Human; MyBioSource
Brand: MyBioSource
SKU: MBS141544
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS141544

Osteopontin is a glycoprotein that was primarilyfound in osteoblasts and takes part in bone remodeling, immune functions in fibroblasts, macrophages, & lymphocytes during inflammation and wound healing. SPP1 highlybinds to hydroxyapatite. SPP1 forms an… More details

$250 / 0.005 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Osteopontin (SPP1), Active Protein

Full Product Name

Recombinant Human Osteopontin, BioActive

Product Gene Name

SPP1 active protein

Product Synonym Names

SPP1 Active; SPP1 Human Active; Osteopontin Human Recombinant; BioActive; OPN; SPP-1; BNSP; BSPI; ETA-1; Bone sialoprotein 1; BSP IEarly T lymphocyte activation 1; ETA 1; ETA1; MGC110940; Nephropontin; Secreted phosphoprotein 1; SPP 1; SPP1; urinary stone protein; uropontin

Sequence

MGSSHHHHHH SSGLVPRGSH RSMIPVKQAD SGSSEEKQLY NKYPDAVATW LNPDPSQKQN LLAPQNAVSS EETNDFKQET LPSKSNESHD HMDDMDDEDD DDHVDSQDSI DSNDSDDVDD TDDSHQSDES HHSDESDELV TDFPTDLPAT EVFTPVVPTV DTYDGRGDSV VYGLRSKSKK FRRPDIQYPD ATDEDITSHM ESEELNGAYK AIPVAQDLNA PSDWDSRGKD SYETSQLDDQ SAETHSHKQS RLYKRKANDE SNEHSDVIDS QELSKVSREF HSHEFHSHED MLVVDPKSKE EDKHLKFRIS HELDSASSEV N

Host

E Coli

Form/Format

Sterile filtered clear colorless solution.
The SPP1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH7.5), 1mM DTT, 10% glycerol and 2mM EDTA.

Purity/Purification

Greater than 90.0% as determined by SDS-PAGE.

Preparation and Storage

Store at 4 degree C if entire vial will be used within 2-4 weeks.
Store, frozen at -20 degree C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Molecular Weight

35,423 Da

Chromosome Location

Chromosome: 4; NC_000004.11 (88896802..88904563). Location: 4q22.1

OMIM

166490

3D Structure

ModBase 3D Structure for P10451

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Biological Activity

Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When cells are added to OPN coated plates 10ug/ml. This effect is more to 40%.

Product Categories/Family for SPP1 active protein

CYTOKINES AND GROWTH FACTORS

Other Notes

Small volumes of SPP1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SPP1 purchase

MBS141544 is an active protein targeting SPP1. Also known as Recombinant Human Osteopontin, BioActive. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for SPP1. It may not necessarily be applicable to this product.

NCBI GI #

4759166

NCBI GeneID

6696

NCBI Accession #

NP_000573.1

UniProt Primary Accession #

P10451

UniProt Secondary Accession #

Q15681; Q15682; Q15683; Q4W597; Q567T5; Q8NBK2; Q96IZ1; B2RDA1

UniProt Related Accession #

P10451

GenBank Nucleotide #

NM_000582.2

NCBI Official Full Name

osteopontin isoform OPN-b

NCBI Official Synonym Full Names

secreted phosphoprotein 1

NCBI Official Symbol

SPP1

NCBI Official Synonym Symbols

OPN; BNSP; BSPI; ETA-1

UniProt Protein Name

Osteopontin

UniProt Synonym Protein Names

Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin

Protein Family

COMPASS component

UniProt Gene Name

SPP1

UniProt Synonym Gene Names

BNSP; OPN; SPP-1

UniProt Entry Name

OSTP_HUMAN

NCBI Protein Information

osteopontin; uropontin; nephropontin; SPP1/CALPHA1 fusion; urinary stone protein; early T-lymphocyte activation 1; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I

NCBI Summary for SPP1

The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

UniProt Comments for SPP1

osteopontin: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Belongs to the osteopontin family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q22.1

Cellular Component: extracellular space; cell projection; perinuclear region of cytoplasm; apical part of cell; extracellular region

Molecular Function: protein binding; extracellular matrix binding; cytokine activity

Biological Process: neutrophil chemotaxis; extracellular matrix organization and biogenesis; decidualization; osteoblast differentiation; extracellular matrix disassembly; response to vitamin D; biomineral formation; response to steroid hormone stimulus; negative regulation of collateral sprouting of intact axon in response to injury; positive regulation of bone resorption; inflammatory response; cell adhesion; embryo implantation

Research Articles on SPP1

1. elevated OPN level in plasma and tumor tissues was significantly associated with metastasis and survival of laryngeal and hypopharyngeal carcinomas.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with SPP1 active protein

Reference Product	PubMed Publications
CD44 active protein	>112 publications with SPP1 and CD44
MMP7 active protein	>8 publications with SPP1 and MMP7
MMP3 active protein	>6 publications with SPP1 and MMP3
ACP5 active protein	>5 publications with SPP1 and ACP5
PRKCA active protein	>3 publications with SPP1 and PRKCA
ITGA5 active protein	>3 publications with SPP1 and ITGA5
IGFBP5 active protein	>3 publications with SPP1 and IGFBP5
ITGB1 active protein	>2 publications with SPP1 and ITGB1
ITGAV active protein	>2 publications with SPP1 and ITGAV
CSNK2A1 active protein	>1 publications with SPP1 and CSNK2A1

Pathways associated with SPP1 active protein

Products by Pathway	Pathway Diagram
BDNF Signaling Pathway antibodies	BDNF Signaling Pathway Diagram
Direct P53 Effectors Pathway antibodies	Direct P53 Effectors Pathway Diagram
ECM-receptor Interaction Pathway antibodies	ECM-receptor Interaction Pathway Diagram
ECM-receptor Interaction Pathway antibodies	ECM-receptor Interaction Pathway Diagram
Endochondral Ossification Pathway antibodies	Endochondral Ossification Pathway Diagram
FGF Signaling Pathway antibodies	FGF Signaling Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Integrin Cell Surface Interactions Pathway antibodies	Integrin Cell Surface Interactions Pathway Diagram

Diseases associated with SPP1 active protein

Disease Name	Pubmed Publications
Neoplasms Antibodies	>479 publications with SPP1 and Neoplasms
Cardiovascular Diseases Antibodies	>241 publications with SPP1 and Cardiovascular Diseases
Kidney Diseases Antibodies	>209 publications with SPP1 and Kidney Diseases
Inflammation Antibodies	>201 publications with SPP1 and Inflammation
Fibrosis Antibodies	>134 publications with SPP1 and Fibrosis
Necrosis Antibodies	>108 publications with SPP1 and Necrosis
Calcinosis Antibodies	>104 publications with SPP1 and Calcinosis
Adenocarcinoma Antibodies	>97 publications with SPP1 and Adenocarcinoma
Heart Diseases Antibodies	>92 publications with SPP1 and Heart Diseases
Breast Neoplasms Antibodies	>79 publications with SPP1 and Breast Neoplasms

Organs/Tissues associated with SPP1 active protein

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>990 publications with SPP1 and Bone
Blood Antibodies	>487 publications with SPP1 and Blood
Kidney Antibodies	>346 publications with SPP1 and Kidney
Connective Tissue Antibodies	>310 publications with SPP1 and Connective Tissue
Vascular Antibodies	>309 publications with SPP1 and Vascular
Muscle Antibodies	>250 publications with SPP1 and Muscle
Bone Marrow Antibodies	>172 publications with SPP1 and Bone Marrow
Mouth Antibodies	>156 publications with SPP1 and Mouth
Heart Antibodies	>117 publications with SPP1 and Heart
Liver Antibodies	>112 publications with SPP1 and Liver