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Marcescens Benzonase Nuclease Serratia Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS141572

Serratia marcescens secretes an endonuclease that has exceptionally high specific activity to the medium that surrounds it. The Benzonase Nuclease is mainly used for elimination of nucleic acid contamination from purified proteins, downstream processing,… More details

$260 / 5000 Units +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Marcescens Benzonase Nuclease, Recombinant Protein
Full Product Name
Recombinant Serratia Marcescens Benzonase Nuclease, 90%
Product Synonym Names
Benzonase Nuclease; 90%; Benzonase Nuclease Serratia Marcescens Recombinant 90%
Host
E Coli
Form/Format
Sterile filtered colorless solution.
The Benzonase Nuclease solution contains 50% glycerol, 50 mM Tris-HCl pH8.0, 20 mM NaCl and 2 mM MgCl2.
Purity/Purification
Greater than 90% as determined by SDS-PAGE.
Specificity
Unspecific (DNA, RNA) attacks all nucleic acids (single strand, double strand, circular, supercoiled) with no apparent sequence preference. Final reaction product:
5'-mono-phosphate terminated oligonucleotides (3-5 bases).
Protease Activity: Not detectable
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks.
Store, frozen at -20 degree C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Unit Definition
1U Benzonase Nuclease is defined as the amount of enzyme that causes a deltaA260 of 1 in 30 min, which corresponds to complete digestion of 37ug DNA. Standard reaction conditions are 1mg/ml sonicated DNA substrate in 50mM Tris-HCl pH8.0, 0.1mg/ml BSA, 1mM MgCl2, incubated at 37 degree C

Other Notes

Small volumes of Marcescens Benzonase Nuclease recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Marcescens Benzonase Nuclease purchase

MBS141572 is a recombinant protein targeting Marcescens Benzonase Nuclease. Also known as Recombinant Serratia Marcescens Benzonase Nuclease, 90%. Host: E Coli.
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