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Thermosensitive Gluconokinase (IDNK) E Coli Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS141600

D-gluconate kinase or idnk is a thermosensitive protein, consists of 187 a. A and part of the gluconokinase gntK/gntV protein family. Idnk enhances the conversion of ATP + D-gluconate => ADP + 6-phospho-D-gluconate. Idnk has a crucial part in determination of… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Thermosensitive Gluconokinase (IDNK), Active Protein
Full Product Name
Recombinant E Coli Thermosensitive Gluconokinase, BioActive
Product Gene Name
Product Synonym Names
IDNK Active; IDNK E Coli Active; Thermosensitive Gluconokinase E Coli Recombinant; BioActive; Thermosensitive gluconokinase; Gluconate kinase 1; idnK; D-gluconate kinase thermosensitive; D-gluconate kinase; thermosensitive; ECK4261; gntV; JW4225; b4268
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAGESFI LMGVSGSGKT LIGSKVAALL SAKFIDGDDL HPAKNIDKMS QGIPLSDEDR LPWLERLNDA SYSLYKKNET GFIVCSSLKK QYRDILRKGS PHVHFLWLDG DYETILARMQ RRAGHFMPVA LLKSQFEALE RPQADEQDIV RIDINHDIAN VTEQCRQAVL AIRQNRICAK EGSASDQRCE
Host
E Coli
Form/Format
Sterile filtered colorless solution.
The IDNK solution (1 mg/ml) contains 10% Glycerol, 1mM DTT, 0.15M NaCl and 20mM Tris-HCl buffer (pH8.0).
Purity/Purification
Greater than 90.0% as determined by SDS-PAGE.
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks.
Store, frozen at -20 degree C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Molecular Weight
23.4 kDa (210aa) confirmed by MALDI-TOF
3D Structure
ModBase 3D Structure for P39208
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Biological Activity
Specific activity is >80unit/mg. One unit will convert 1.0 umole of D-gluconate to 6-phospho-Dgluconate per minute at pH8.0 at 37 degree C

Product Categories/Family for IDNK active protein

Other Notes

Small volumes of IDNK active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IDNK purchase

MBS141600 is an active protein targeting IDNK. Also known as Recombinant E Coli Thermosensitive Gluconokinase, BioActive. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for IDNK. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
GenBank Nucleotide #
NCBI Official Full Name
D-gluconate kinase, thermosensitive
NCBI Official Symbol
idnK
NCBI Official Synonym Symbols
ECK4261; gntV; JW4225
UniProt Protein Name
Thermosensitive gluconokinase
UniProt Synonym Protein Names
Gluconate kinase 1
UniProt Gene Name
idnK
UniProt Synonym Gene Names
gntV
NCBI Protein Information
D-gluconate kinase, thermosensitive

NCBI Summary for IDNK

The subunit structure is unknown, however gluconokinase I is known to be a homotrimer. [More information is available at EcoCyc: EG12152].

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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