Human NME2 Active Protein – Cat# MBS141636

Non-Metastatic Cells 2 (NME2) Human Active Protein

Name: NME2 Active Protein; Non-Metastatic Cells 2; Human; MyBioSource
Brand: MyBioSource
SKU: MBS141636
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS141636

Non-Metastatic Cells 2 or NME2, is a protein, that acts as a nucleoside diphosphate kinase. This protein is of a heterodimeric structure and has 152 aa A & B polypeptide chains that builds the whole protein. Erythrocyte NDP kinase beta subunit has an… More details

$250 / 0.002 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Non-Metastatic Cells 2 (NME2), Active Protein

Full Product Name

Recombinant Human Non-Metastatic Cells 2

Product Gene Name

NME2 active protein

Product Synonym Names

NME2; NME2 Human; Non-Metastatic Cells 2 Human Recombinant; Nucleoside diphosphate kinase B; NDPK-B; NDPKB; NM23-H2; NM23B

Sequence

MANLERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVKSAEKEI SLWFKPEELV DYKSCAHDWV YE

Host

E Coli

Form/Format

Sterile filtered colorless solution.
The NME2 solution (1mg/ml) contains 10% glycerol, 20mM Tris-HCl buffer (pH8.0) and 1mM DTT.

Purity/Purification

Greater than 90.0% as determined by SDS-PAGE.

Preparation and Storage

Store at 4 degree C if entire vial will be used within 2-4 weeks.
Store, frozen at -20 degree C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.

Molecular Weight

17,298 Da

Chromosome Location

Chromosome: 17; NC_000017.10 (49230897..49249105). Location: 17q21.3

OMIM

156491

3D Structure

ModBase 3D Structure for P22392

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Biological Activity

Specific activity is >1,800unit/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH7.5 at 25 degree C in a couple system with PK/LDH.

Product Categories/Family for NME2 active protein

RECOMBINANT & NATURAL PROTEINS

Other Notes

Small volumes of NME2 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NME2 purchase

MBS141636 is an active protein targeting NME2. Also known as Recombinant Human Non-Metastatic Cells 2. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for NME2. It may not necessarily be applicable to this product.

NCBI GI #

66392203

NCBI GeneID

654364

NCBI Accession #

NP_001018146.1

UniProt Primary Accession #

P22392

UniProt Secondary Accession #

Q1WM23; Q6LCT6; A8MWA3

GenBank Nucleotide #

NM_001018136.2

NCBI Official Full Name

NME1-NME2 protein

NCBI Official Synonym Full Names

NME1-NME2 readthrough

NCBI Official Symbol

NME1-NME2

NCBI Official Synonym Symbols

NMELV; NM23-LV

UniProt Protein Name

Nucleoside diphosphate kinase B

UniProt Synonym Protein Names

C-myc purine-binding transcription factor PUF; Histidine protein kinase NDKB (EC:2.7.13.3); nm23-H2

UniProt Gene Name

NME2

UniProt Synonym Gene Names

NM23B; NDK B; NDP kinase B

UniProt Entry Name

NDKB_HUMAN

NCBI Protein Information

NME1-NME2 protein; NME1-NME2 readthrough transcript

NCBI Summary for NME2

This locus represents naturally occurring read-through transcription between the neighboring NME1 and NME2 genes. The significance of this read-through transcription and the function of the resulting protein product have not yet been determined. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2010]

UniProt Comments for NME2

NME2: Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity. Belongs to the NDK family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, other; Nucleotide Metabolism - pyrimidine; EC 2.7.13.3; Nucleotide Metabolism - purine; Nuclear receptor co-regulator; EC 2.7.4.6; Kinase, nucleoside diphosphate; Other group; NDK family

Chromosomal Location of Human Ortholog: 17q21.3

Cellular Component: ruffle; centrosome; focal adhesion; membrane; mitochondrion; perinuclear region of cytoplasm; lamellipodium; cytoplasm; intermediate filament; cytosol; nucleus

Molecular Function: gamma-tubulin binding; protein binding; protein histidine kinase activity; metal ion binding; nucleoside diphosphate kinase activity; intermediate filament binding; protein kinase binding; transcription factor activity; ATP binding; single-stranded DNA binding

Biological Process: integrin-mediated signaling pathway; GTP biosynthetic process; peptidyl-histidine phosphorylation; response to cAMP; transcription, DNA-dependent; nucleobase, nucleoside and nucleotide interconversion; nucleobase, nucleoside and nucleotide metabolic process; negative regulation of myeloid leukocyte differentiation; UTP biosynthetic process; hippocampus development; nucleoside diphosphate phosphorylation; response to testosterone stimulus; CTP biosynthetic process; regulation of transcription, DNA-dependent; pyrimidine nucleotide metabolic process; nucleoside triphosphate biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; cell adhesion; positive regulation of epithelial cell proliferation; regulation of epidermis development; response to amine stimulus; positive regulation of keratinocyte differentiation; purine nucleotide metabolic process; negative regulation of apoptosis

Research Articles on NME2

1. Observational study of gene-disease association. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with NME2 active protein

Products by Pathway	Pathway Diagram
Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway antibodies	Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway Diagram
Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway antibodies	Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway Diagram
CMP Phosphorylation Pathway antibodies	CMP Phosphorylation Pathway Diagram
Guanine Ribonucleotide Biosynthesis IMP => GDP,GTP Pathway antibodies	Guanine Ribonucleotide Biosynthesis IMP => GDP,GTP Pathway Diagram
Guanine Ribonucleotide Biosynthesis IMP => GDP,GTP Pathway antibodies	Guanine Ribonucleotide Biosynthesis IMP => GDP,GTP Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Nucleotides Pathway antibodies	Metabolism Of Nucleotides Pathway Diagram
Nucleotide Metabolism Pathway antibodies	Nucleotide Metabolism Pathway Diagram
Purine Metabolism Pathway antibodies	Purine Metabolism Pathway Diagram
Purine Metabolism Pathway antibodies	Purine Metabolism Pathway Diagram

Diseases associated with NME2 active protein

Disease Name	Pubmed Publications
Lung Neoplasms Antibodies	>1 publications with NME2 and Lung Neoplasms
Neoplasm Metastasis Antibodies	>1 publications with NME2 and Neoplasm Metastasis
Anemia Antibodies	>1 publications with NME2 and Anemia
Carcinoma, Hepatocellular Antibodies	>1 publications with NME2 and Carcinoma, Hepatocellular

Organs/Tissues associated with NME2 active protein

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>1 publications with NME2 and Lung