Human MIP 1a Active Protein – Cat# MBS142009

Macrophage Inflammatory Protein-1 Alpha (MIP 1a) Human Active Protein

Name: MIP 1a Active Protein; Human; MyBioSource
Brand: MyBioSource
SKU: MBS142009
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS142009

Description: Macrophage Inflammatory Protein-1 alpha Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7820 Dalton. The MIP-1a is purified by proprietary… More details

$250 / 0.005 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Macrophage Inflammatory protein-1 alpha (MIP 1a), Active Protein

Full Product Name

Recombinant Human Macrophage Inflammatory protein-1 alpha (CCL3)

Product Gene Name

MIP 1a active protein

Product Synonym Names

MIP 1a Human; Macrophage Inflammatory Protein-1 Alpha Human Recombinant (CCL3); Small inducible cytokine A3; CCL3; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; Tonsillar lymphocyte LD78 alpha protein; G0/G1 switch regulatory protein 19-1; G0S19-1 protein; SIS-beta; PAT 464.1; chemokine (C-C motif) ligand 3; MIP1A; SCYA3; G0S19-1; LD78ALPHA

Sequence

The sequence of the first five N-terminal amino acids was determined and was found to be, Ala-Ser-Le u-Ala-Ala

Host

E Coli

Form/Format

The protein was lyophilized from 0.55 mg/ml solution containing no additives.
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity/Purification

Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Preparation and Storage

Lyophilized MIP-1a although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution CCL3 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Molecular Weight

10,085 Da

OMIM

182283

3D Structure

ModBase 3D Structure for P10147

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility

It is recommended to reconstitute the lyophilized Macrophage Inflammatory Protein-1a in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.

Biological Activity

The Activity is calculated by the ability of chemo-attraction of Human monocytes using 1-10ng/ml corresponding to a Specific Activity of 100,000-1,000,000IU/mg.

Product Categories/Family for MIP 1a active protein

Chemokines; MIP

Other Notes

Small volumes of MIP 1a active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MIP 1a purchase

MBS142009 is an active protein targeting MIP 1a. Also known as Recombinant Human Macrophage Inflammatory protein-1 alpha (CCL3). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for MIP 1a. It may not necessarily be applicable to this product.

NCBI GI #

4506843

NCBI GeneID

6348

NCBI Accession #

NP_002974.1

UniProt Primary Accession #

P10147

UniProt Related Accession #

P10147

GenBank Nucleotide #

NM_002983.2

NCBI Official Full Name

C-C motif chemokine 3

NCBI Official Synonym Full Names

chemokine (C-C motif) ligand 3

NCBI Official Symbol

CCL3

NCBI Official Synonym Symbols

MIP1A; SCYA3; G0S19-1; LD78ALPHA; MIP-1-alpha

UniProt Protein Name

C-C motif chemokine 3

UniProt Synonym Protein Names

G0/G1 switch regulatory protein 19-1; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; PAT 464.1; SIS-beta; Small-inducible cytokine A3; Tonsillar lymphocyte LD78 alpha proteinMIP-1-alpha(4-69)Alternative name(s):LD78-alpha(4-69)

Protein Family

C-C motif chemokine

UniProt Gene Name

CCL3

UniProt Synonym Gene Names

G0S19-1; MIP1A; SCYA3; MIP-1-alpha

UniProt Entry Name

CCL3_HUMAN

NCBI Protein Information

C-C motif chemokine 3; G0/G1 switch regulatory protein 19-1; PAT 464.1; SIS-beta; macrophage inflammatory protein 1-alpha; small inducible cytokine A3 (homologous to mouse Mip-1a); tonsillar lymphocyte LD78 alpha protein

NCBI Summary for MIP 1a

This locus represents a small inducible cytokine. The encoded protein, also known as macrophage inflammatory protein 1 alpha, plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. Polymorphisms at this locus may be associated with both resistance and susceptibility to infection by human immunodeficiency virus type 1.[provided by RefSeq, Sep 2010]

UniProt Comments for MIP 1a

CCL3: Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide; Chemokine

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space; cytoplasm; extracellular region; intracellular; cytosol

Molecular Function: identical protein binding; protein binding; CCR1 chemokine receptor binding; chemokine activity; calcium-dependent protein kinase C activity; kinase activity; chemoattractant activity; CCR5 chemokine receptor binding; protein kinase activity; phospholipase activator activity

Biological Process: positive regulation of catalytic activity; negative regulation of osteoclast differentiation; exocytosis; behavior; response to toxin; positive regulation of calcium-mediated signaling; positive regulation of interleukin-1 beta secretion; chemotaxis; protein amino acid phosphorylation; monocyte chemotaxis; regulation of cell shape; negative regulation of bone mineralization; positive chemotaxis; cell-cell signaling; positive regulation of neuron apoptosis; calcium ion transport; protein kinase B signaling cascade; lipopolysaccharide-mediated signaling pathway; inflammatory response; lymphocyte chemotaxis; neutrophil chemotaxis; MAPKKK cascade; calcium-mediated signaling; cytoskeleton organization and biogenesis; positive regulation of tumor necrosis factor production; release of sequestered calcium ion by sarcoplasmic reticulum into cytosol; macrophage chemotaxis; cellular calcium ion homeostasis; osteoblast differentiation; cell activation; positive regulation of protein kinase B signaling cascade; eosinophil chemotaxis; eosinophil degranulation; regulation of sensory perception of pain; positive regulation of calcium ion transport; astrocyte cell migration; positive regulation of inflammatory response; positive regulation of cell migration

Disease: Human Immunodeficiency Virus Type 1, Susceptibility To

Research Articles on MIP 1a

1. MIP-1alpha levels in Jurkat cells appeared to be an important factor for its transendothelial migration

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MIP 1a active protein

Reference Product	PubMed Publications
CCL4 active protein	>1620 publications with MIP 1a and CCL4
CCR5 active protein	>507 publications with MIP 1a and CCR5
CCR1 active protein	>280 publications with MIP 1a and CCR1
CCR2 active protein	>137 publications with MIP 1a and CCR2
CCR3 active protein	>68 publications with MIP 1a and CCR3
CCR4 active protein	>46 publications with MIP 1a and CCR4
IL18 active protein	>20 publications with MIP 1a and IL18
IL1B active protein	>15 publications with MIP 1a and IL1B
IDE active protein	>5 publications with MIP 1a and IDE
CXCR5 active protein	>4 publications with MIP 1a and CXCR5

Pathways associated with MIP 1a active protein

Products by Pathway	Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram
Chemokine Receptors Bind Chemokines Pathway antibodies	Chemokine Receptors Bind Chemokines Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies	Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram

Diseases associated with MIP 1a active protein

Disease Name	Pubmed Publications
Inflammation Antibodies	>893 publications with MIP 1a and Inflammation
Necrosis Antibodies	>506 publications with MIP 1a and Necrosis
Disease Models, Animal Antibodies	>314 publications with MIP 1a and Disease Models, Animal
Lung Diseases Antibodies	>238 publications with MIP 1a and Lung Diseases
HIV Infections Antibodies	>188 publications with MIP 1a and HIV Infections
Cardiovascular Diseases Antibodies	>173 publications with MIP 1a and Cardiovascular Diseases
Hypersensitivity Antibodies	>105 publications with MIP 1a and Hypersensitivity
Liver Diseases Antibodies	>95 publications with MIP 1a and Liver Diseases
Fibrosis Antibodies	>79 publications with MIP 1a and Fibrosis
Pneumonia Antibodies	>72 publications with MIP 1a and Pneumonia

Organs/Tissues associated with MIP 1a active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>1553 publications with MIP 1a and Blood
Lung Antibodies	>328 publications with MIP 1a and Lung
Brain Antibodies	>257 publications with MIP 1a and Brain
Liver Antibodies	>216 publications with MIP 1a and Liver
Kidney Antibodies	>85 publications with MIP 1a and Kidney
Heart Antibodies	>79 publications with MIP 1a and Heart
Intestine Antibodies	>56 publications with MIP 1a and Intestine
Eye Antibodies	>51 publications with MIP 1a and Eye
Uterus Antibodies	>19 publications with MIP 1a and Uterus
Pancreas Antibodies	>18 publications with MIP 1a and Pancreas