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Adenylyltransferase and Sulfurtransferase Uba4 (uba4) Neosartorya Fumigata Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1420235

MBS1420235 is a recombinant protein targeting uba4. Also known as Recombinant Neosartorya fumigata Adenylyltransferase and sulfurtransferase uba4 (uba4). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

$1290 / 0.05 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Adenylyltransferase and sulfurtransferase uba4 (uba4), Recombinant Protein
Full Product Name
Recombinant Neosartorya fumigata Adenylyltransferase and sulfurtransferase uba4 (uba4)
Product Gene Name
Product Synonym Gene Name
uba4; cnxF; AFUA_5G10480
Product Synonym Names
Adenylyltransferase and sulfurtransferase uba4; Common component for nitrate reductase and xanthine dehydrogenase protein F; Ubiquitin-like protein activator 4; Including the following 2 domains:; Molybdopterin-synthase adenylyltransferase; EC=2.7.7.80; A
Sequence
MENLEQTCAS LRAQIAATEA QLAGLKRELE IAEQAAEVKA QSTTRTITAE DGKTNETREW PLLSEEYKRY GRQMIVPQLG LQGQLKLRAA RVLIVGAGGL GCPAALYLAG AGVGTLGLVD GDTVENSNLH RQVLHRSKNV GTFKVDSAIE YLRELNPHPT YVPYRAHLTP QEAPGIFKDY DIVLDCTDNP ATRYLISDTA VLLGKPLVSA SALRTEGQLM VLNYPPRPVG DKSGGPCYRC VFPKPPPANS VVSCADGGIL GPVVGTMGVL QALEAIKVIT SPAVNPSASP PSLLIFSAYS TPLFRTIRLR ARRANCAVCS ADASVTLETL KNGSTDYVFF CGVAGLEATL SPEERISPLE FKKRHPKEVP QDGGRINKEP TIIDVREKVQ FDICSLENSI NIPISTILSS ASSPTNVDAN AQPSLPFWLP RELASADSTD PIYVVCRHGN DSQIAVRRLK ELGLDRGGQR YVGDIQGGLR AWREQIDPDW PEY
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
Molecular Weight
53,454 Da
Sequence Positions
1-493aa; full length protein
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for Q4WV19
Species
Neosartorya fumigata (strain MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of uba4 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for uba4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
molybdenum cofactor biosynthetic protein (CnxF)
NCBI Official Symbol
AFUA_5G10480
UniProt Protein Name
Adenylyltransferase and sulfurtransferase uba4
UniProt Synonym Protein Names
Common component for nitrate reductase and xanthine dehydrogenase protein F; Ubiquitin-like protein activator 4; ; Adenylyltransferase uba4; Sulfur carrier protein MOCS2A adenylyltransferase; ; Sulfur carrier protein MOCS2A sulfurtransferase; Sulfurtransferase uba4
UniProt Gene Name
uba4
UniProt Synonym Gene Names
cnxF; AFUA_5G10480
UniProt Entry Name
UBA4_ASPFU
NCBI Protein Information
molybdenum cofactor biosynthetic protein (CnxF), putative

UniProt Comments for uba4

Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers urm1 and mocs2a. Its N-terminus first activates urm1 and mocs2a as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to urm1 and mocs2a to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards urm1 and mocs2a. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; nfs1 probably acting as a sulfur donor for thiocarboxylation reactions ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with uba4 recombinant protein

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