Human ENA 78 Active Protein – Cat# MBS142038

Epithelial Neutrophil-Activating Protein 78 (ENA 78) Human Active Protein

Name: ENA 78 Active Protein; Human; MyBioSource
Brand: MyBioSource
SKU: MBS142038
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS142038

Description: Epithelial Neutrophil-Activating Protein 78 Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 74 amino acids and having a molecular mass of 8020 Dalton. The CXCL5 is purified by proprietary… More details

$250 / 0.005 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Epithelial Neutrophil-Activating Protein 78 (ENA 78), Active Protein

Full Product Name

Recombinant Human Epithelial Neutrophil-Activating Protein 78 (CXCL5)

Product Gene Name

ENA 78 active protein

Product Synonym Names

ENA 78 Human; Epithelial Neutrophil-Activating Protein 78 Human Recombinant (CXCL5); Small inducible cytokine B5; CXCL5; Epithelial-derived neutrophil-activating protein 78; Neutrophil-activating peptide ENA-78; ENA-78(1-78); chemokine (C-X-C motif) ligand 5; SCYB5

Sequence

The sequence of the first five N-terminal amino acids was determined and was found to be, Ala- Ala -Val-Leu-A rg

Host

E Coli

Form/Format

The CXCL5 was lyophilized from a concentrated (1mg/ml) solution in water containing no additives.
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity/Purification

Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Preparation and Storage

Lyophilized ENA78 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution CXCL5 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Molecular Weight

11,972 Da

OMIM

600324

3D Structure

ModBase 3D Structure for P42830

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility

It is recommended to reconstitute the lyophilized ENA-78 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.

Biological Activity

The biological activity was determined by measuring the dose dependent mobilization of intracellular calcium (calcium flux) with human neutrophils. Significant calcium mobilization is observed with 100ng/mL (corresponding to a Specific Activity of 10,000IU/mg) of recombinant human ENA-78.

Product Categories/Family for ENA 78 active protein

Chemokines; ENA 78

Other Notes

Small volumes of ENA 78 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ENA 78 purchase

MBS142038 is an active protein targeting ENA 78. Also known as Recombinant Human Epithelial Neutrophil-Activating Protein 78 (CXCL5). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for ENA 78. It may not necessarily be applicable to this product.

NCBI GI #

4506849

NCBI GeneID

6374

NCBI Accession #

NP_002985.1

UniProt Primary Accession #

P42830

UniProt Secondary Accession #

Q96QE1

UniProt Related Accession #

P42830

GenBank Nucleotide #

NM_002994.4

NCBI Official Full Name

C-X-C motif chemokine 5

NCBI Official Synonym Full Names

chemokine (C-X-C motif) ligand 5

NCBI Official Symbol

CXCL5

NCBI Official Synonym Symbols

SCYB5; ENA-78

UniProt Protein Name

C-X-C motif chemokine 5

UniProt Synonym Protein Names

ENA-78(1-78); Epithelial-derived neutrophil-activating protein 78; Neutrophil-activating peptide ENA-78; Small-inducible cytokine B5Cleaved into the following 2 chains:ENA-78(8-78); ENA-78(9-78)

UniProt Gene Name

CXCL5

UniProt Synonym Gene Names

ENA78; SCYB5

UniProt Entry Name

CXCL5_HUMAN

NCBI Protein Information

C-X-C motif chemokine 5; epithelial-derived neutrophil-activating protein 78; neutrophil-activating peptide ENA-78; small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78)

NCBI Summary for ENA 78

This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils, to promote angiogenesis and to remodel connective tissues. This protein is thought to play a role in cancer cell proliferation, migration, and invasion. [provided by RefSeq, May 2013]

UniProt Comments for ENA 78

CXCL5: Involved in neutrophil activation. In vitro, ENA-78(8- 78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine activity; CXCR chemokine receptor binding

Biological Process: G-protein coupled receptor protein signaling pathway; cell-cell signaling; positive regulation of cell proliferation; neutrophil mediated immunity; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; immune response; signal transduction; inflammatory response; chemotaxis

Research Articles on ENA 78

1. Solution structure of CXCL5--a novel chemokine and adipokine implicated in inflammation and obesity

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ENA 78 active protein

Reference Product	PubMed Publications
CXCR2 active protein	>88 publications with ENA 78 and CXCR2
CCL5 active protein	>74 publications with ENA 78 and CCL5
CCL20 active protein	>42 publications with ENA 78 and CCL20
CXCR1 active protein	>26 publications with ENA 78 and CXCR1
CXCL11 active protein	>21 publications with ENA 78 and CXCL11
CXCL16 active protein	>11 publications with ENA 78 and CXCL16
CCR3 active protein	>8 publications with ENA 78 and CCR3
CCR1 active protein	>8 publications with ENA 78 and CCR1
CCL19 active protein	>8 publications with ENA 78 and CCL19
CCR5 active protein	>8 publications with ENA 78 and CCR5

Pathways associated with ENA 78 active protein

Products by Pathway	Pathway Diagram
Chemokine Receptors Bind Chemokines Pathway antibodies	Chemokine Receptors Bind Chemokines Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies	Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
G Alpha (i) Signalling Events Pathway antibodies	G Alpha (i) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram

Diseases associated with ENA 78 active protein

Disease Name	Pubmed Publications
Inflammation Antibodies	>240 publications with ENA 78 and Inflammation
Respiratory Tract Diseases Antibodies	>102 publications with ENA 78 and Respiratory Tract Diseases
Necrosis Antibodies	>102 publications with ENA 78 and Necrosis
Lung Diseases Antibodies	>92 publications with ENA 78 and Lung Diseases
Disease Models, Animal Antibodies	>65 publications with ENA 78 and Disease Models, Animal
Carcinoma Antibodies	>60 publications with ENA 78 and Carcinoma
Cardiovascular Diseases Antibodies	>45 publications with ENA 78 and Cardiovascular Diseases
Fibrosis Antibodies	>28 publications with ENA 78 and Fibrosis
Lung Neoplasms Antibodies	>27 publications with ENA 78 and Lung Neoplasms
Pneumonia Antibodies	>27 publications with ENA 78 and Pneumonia

Organs/Tissues associated with ENA 78 active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>307 publications with ENA 78 and Blood
Lung Antibodies	>135 publications with ENA 78 and Lung
Vascular Antibodies	>98 publications with ENA 78 and Vascular
Intestine Antibodies	>34 publications with ENA 78 and Intestine
Skin Antibodies	>31 publications with ENA 78 and Skin
Kidney Antibodies	>17 publications with ENA 78 and Kidney
Brain Antibodies	>17 publications with ENA 78 and Brain
Connective Tissue Antibodies	>16 publications with ENA 78 and Connective Tissue
Embryonic Tissue Antibodies	>14 publications with ENA 78 and Embryonic Tissue
Uterus Antibodies	>13 publications with ENA 78 and Uterus