Mouse mGRO1/KC Active Protein – Cat# MBS142042

GRO/Kc (mGRO1/KC) Mouse Active Protein

Name: mGRO1/KC Active Protein; GRO/Kc; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS142042
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS142042

Description: KC Mouse Recombinant also known as N51 and GRO1 produced in E Coli is a single, non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of approximately 8 kDa.The GRO-1 is purified by proprietary chromatographic… More details

$250 / 0.005 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

GRO/KC (mGRO1/KC), Active Protein

Full Product Name

Recombinant Mouse GRO/KC (CXCL1)

Product Gene Name

mGRO1/KC active protein

Product Synonym Names

GRO1/KC Mouse; GRO1/KC Mouse Recombinant (CXCL1); Growth-regulated alpha protein; CXCL1; Platelet-derived growth factor-inducible protein KC; Secretory protein N51; KC; Fsp; N51; gro; Gro1; Mgsa; Scyb1; chemokine (C-X-C motif) ligand 1

Sequence

RLATGAPIAN ELRCQ CLQTMAGIHL KNIQSLKVLP SGPHCTQTEV IATLKNGREA CLDPEAPLVQ KIVQKMLKGV PK

Host

E Coli

Form/Format

The protein was lyophilized with no additives.
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity/Purification

Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Preparation and Storage

Lyophilized KC Mouse although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution CXCL1 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Molecular Weight

10,254 Da

3D Structure

ModBase 3D Structure for P12850

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility

It is recommended to reconstitute the lyophilized GRO1 Recombinant in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.

Protein Content

Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.03 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of KC as a Reference Standard.

Biological Activity

The biological activity was determined by measuring the dose dependent mobilization of intracellular calcium (calcium flux) with human neutrophils. Significant calcium mobilization is observed with ?50ng/mL of recombinant mouse KC (Specific Activity: ? 2 x 104 units/mg).

Product Categories/Family for mGRO1/KC active protein

Chemokines; GRO

Other Notes

Small volumes of mGRO1/KC active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for mGRO1/KC purchase

MBS142042 is an active protein targeting mGRO1/KC. Also known as Recombinant Mouse GRO/KC (CXCL1). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for mGRO1/KC. It may not necessarily be applicable to this product.

NCBI GI #

6680109

NCBI GeneID

14825

NCBI Accession #

NP_032202.1

UniProt Primary Accession #

P12850

UniProt Related Accession #

P12850

GenBank Nucleotide #

NM_008176.3

NCBI Official Full Name

growth-regulated alpha protein

NCBI Official Synonym Full Names

chemokine (C-X-C motif) ligand 1

NCBI Official Symbol

Cxcl1

NCBI Official Synonym Symbols

KC; Fsp; N51; gro; Gro1; Mgsa; Scyb1

UniProt Protein Name

Growth-regulated alpha protein

UniProt Synonym Protein Names

C-X-C motif chemokine 1; Platelet-derived growth factor-inducible protein KC; Secretory protein N51KC(5-72)Alternative name(s):Hematopoietic synergistic factor; HSF; KC-T

UniProt Gene Name

Cxcl1

UniProt Synonym Gene Names

Gro; Gro1; Mgsa; Scyb1; HSF

UniProt Entry Name

GROA_MOUSE

NCBI Protein Information

growth-regulated alpha protein; C-X-C motif chemokine 1; GRO1 oncogene; KC/GR)-alpha; KC/GRO-alpha; alpha-chemokine; platelet-derived growth factor-inducible protein KC; secretory protein N51

NCBI Summary for mGRO1/KC

This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This secretory protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils. In mouse, deficiency of this gene is associated with colitis and with defects in immune cell recruitment to the lung. [provided by RefSeq, Apr 2013]

UniProt Comments for mGRO1/KC

CXCL2: Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Cellular Component: extracellular space; cell; extracellular region; cytosol

Molecular Function: growth factor activity; chemokine activity; CXCR chemokine receptor binding; cytokine activity

Biological Process: G-protein coupled receptor protein signaling pathway; neutrophil chemotaxis; elevation of cytosolic calcium ion concentration; positive regulation of superoxide release; response to molecule of bacterial origin; positive regulation of potassium ion transport; immune response; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; inflammatory response; acute inflammatory response; regulation of cell proliferation

Research Articles on mGRO1/KC

1. phagocytosis of refrigerator-stored RBCs in vivo induced increases in circulating monocyte chemoattractant protein-1 (MCP-1) and keratinocyte chemoattractant (KC)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with mGRO1/KC active protein

Products by Pathway	Pathway Diagram
Chemokine Receptors Bind Chemokines Pathway antibodies	Chemokine Receptors Bind Chemokines Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokines And Inflammatory Response (BioCarta) Pathway antibodies	Cytokines And Inflammatory Response (BioCarta) Pathway Diagram
Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies	Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram

Diseases associated with mGRO1/KC active protein

Disease Name	Pubmed Publications
Inflammation Antibodies	>1183 publications with mGRO1/KC and Inflammation
Necrosis Antibodies	>568 publications with mGRO1/KC and Necrosis
Disease Models, Animal Antibodies	>434 publications with mGRO1/KC and Disease Models, Animal
Lung Diseases Antibodies	>337 publications with mGRO1/KC and Lung Diseases
Cardiovascular Diseases Antibodies	>198 publications with mGRO1/KC and Cardiovascular Diseases
Lung Injury Antibodies	>174 publications with mGRO1/KC and Lung Injury
Pneumonia Antibodies	>116 publications with mGRO1/KC and Pneumonia
Wounds and Injuries Antibodies	>98 publications with mGRO1/KC and Wounds and Injuries
Reperfusion Injury Antibodies	>97 publications with mGRO1/KC and Reperfusion Injury
Fibrosis Antibodies	>91 publications with mGRO1/KC and Fibrosis

Organs/Tissues associated with mGRO1/KC active protein

Organ/Tissue Name	Pubmed Publications
Connective Tissue Antibodies	>65 publications with mGRO1/KC and Connective Tissue
Embryonic Tissue Antibodies	>46 publications with mGRO1/KC and Embryonic Tissue
Spinal Cord Antibodies	>33 publications with mGRO1/KC and Spinal Cord
Adipose Tissue Antibodies	>24 publications with mGRO1/KC and Adipose Tissue
Ovary Antibodies	>17 publications with mGRO1/KC and Ovary
Mammary Gland Antibodies	>10 publications with mGRO1/KC and Mammary Gland
Extraembryonic Tissue Antibodies	>1 publications with mGRO1/KC and Extraembryonic Tissue
Testis Antibodies	>1 publications with mGRO1/KC and Testis