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Granulocyte Macrophage-Colony Stimulating Factor (mGM CSF) Mouse Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS142099

Description: Granulocyte Macrophage Colony Stimulating Factor Mouse Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 125 amino acids and having a molecular mass of 14285.35 Dalton.GM-CSF Mouse is purified by… More details

$250 / 0.005 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Granulocyte Macrophage-Colony Stimulating Factor (mGM CSF), Active Protein
Full Product Name
Recombinant Mouse Granulocyte Macrophage-Colony Stimulating Factor
Product Gene Name
mGM CSF active protein
Product Synonym Names
GM CSF Mouse; Granulocyte Macrophage-Colony Stimulating Factor Mouse Recombinant; CSF-2; MGI-1GM; GM-CSF; Pluripoietin-alpha; Molgramostin; Sargramostim
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pr o-Thr-Arg
Form/Format
GM-CSF Mouse was lyophilized with no additives.
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity/Purification
Greater than 98.0% as determined by
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Preparation and Storage
Lyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution GM-CSF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Molecular Weight
16,091 Da
3D Structure
ModBase 3D Structure for P01587
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Source
E Coli
Solubility
It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 20mM AcOH (acetic Acid) not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Protein Content
GM-CSF quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.765 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of GM-CSF as a Reference Standard.
Biological Activity
The ED50 as determined by the dose-dependant stimulation of the proliferation of murine FDC-P1 cell line is < 0.2 ng/ml, corresponding to a Specific Activity of 5 x 106 IU/mg.

Product Categories/Family for mGM CSF active protein

Cytokines and Growth Factors; Growth Factors; Colony Stimulating Factor

Other Notes

Small volumes of mGM CSF active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for mGM CSF purchase

MBS142099 is an active protein targeting mGM CSF. Also known as Recombinant Mouse Granulocyte Macrophage-Colony Stimulating Factor.

Additional Details

NCBI/Uniprot data below describe general gene information for mGM CSF. It may not necessarily be applicable to this product.

NCBI GI #
110625658
NCBI GeneID
12981
NCBI Accession #
NP_034099.2
UniProt Primary Accession #
P01587
UniProt Related Accession #
P01587
GenBank Nucleotide #
NM_009969.4
NCBI Official Full Name
granulocyte-macrophage colony-stimulating factor
NCBI Official Synonym Full Names
colony stimulating factor 2 (granulocyte-macrophage)
NCBI Official Symbol
Csf2
NCBI Official Synonym Symbols
Csfgm; GMCSF; Gm-CSf; MGI-IGM
UniProt Protein Name
Granulocyte-macrophage colony-stimulating factor
UniProt Synonym Protein Names
Colony-stimulating factor; CSF
Protein Family
Granulocyte-macrophage colony-stimulating factor
UniProt Gene Name
Csf2
UniProt Synonym Gene Names
Csfgm; GM-CSF; CSF
UniProt Entry Name
CSF2_MOUSE
NCBI Protein Information
granulocyte-macrophage colony-stimulating factor; CSF; granulocyte-macrophage colony stimulating factor 2; put. GM-CSF

UniProt Comments for mGM CSF

GMCSF: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Belongs to the GM-CSF family.

Protein type: Secreted; Cytokine; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region

Molecular Function: growth factor activity; granulocyte macrophage colony-stimulating factor receptor binding; cytokine activity

Biological Process: negative regulation of cytolysis; epithelial fluid transport; embryonic placenta development; macrophage activation; positive regulation of tyrosine phosphorylation of Stat5 protein; regulation of gene expression; positive regulation of interleukin-23 production; positive regulation of cell proliferation; myeloid dendritic cell differentiation; immune response; positive regulation of DNA replication; regulation of cell proliferation
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Research Articles on mGM CSF

1. Expression of PPAR-gamma in fetal lung monocytes was dependent on the cytokine GM-CSF. Therefore, GM-CSF has a lung-specific role in the perinatal development of alveolar macrophages through the induction of PPAR-gamma in fetal monocytes.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with mGM CSF active protein

 Products by Pathway  Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokines And Inflammatory Response (BioCarta) Pathway antibodies  Cytokines And Inflammatory Response (BioCarta) Pathway Diagram
 Fc Epsilon RI Signaling Pathway antibodies  Fc Epsilon RI Signaling Pathway Diagram
 Fc Epsilon RI Signaling Pathway antibodies  Fc Epsilon RI Signaling Pathway Diagram
 G Beta:gamma Signalling Through PI3Kgamma Pathway antibodies  G Beta:gamma Signalling Through PI3Kgamma Pathway Diagram
 G-protein Beta:gamma Signalling Pathway antibodies  G-protein Beta:gamma Signalling Pathway Diagram

Diseases associated with mGM CSF active protein

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >41 publications with mGM CSF and Inflammation
 Necrosis Antibodies  >26 publications with mGM CSF and Necrosis
 Lung Diseases Antibodies  >9 publications with mGM CSF and Lung Diseases
 Breast Neoplasms Antibodies  >7 publications with mGM CSF and Breast Neoplasms
 Fibrosis Antibodies  >4 publications with mGM CSF and Fibrosis
 Neoplasms, Experimental Antibodies  >4 publications with mGM CSF and Neoplasms, Experimental
 Carcinoma Antibodies  >3 publications with mGM CSF and Carcinoma
 Leukemia, Myeloid, Acute Antibodies  >3 publications with mGM CSF and Leukemia, Myeloid, Acute
 Arthritis, Rheumatoid Antibodies  >3 publications with mGM CSF and Arthritis, Rheumatoid
 Lung Neoplasms Antibodies  >3 publications with mGM CSF and Lung Neoplasms

Organs/Tissues associated with mGM CSF active protein

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >12 publications with mGM CSF and Lung
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