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Leptin Triple Antagonist Human Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS142229

Description: Leptin Antagonist Triple Mutant Human Recombinant is a single non-glycosilated polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular weight of 16 kDa, Leptin was mutated, resulting in L39A/D40A/F41A.… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
Leptin Triple Antagonist, Active Protein
Full Product Name
Recombinant Human Leptin Triple Antagonist
Product Synonym Names
Leptin tA Human; Leptin Antagonist Triple Mutant Human Recombinant; Leptin tA
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pr o-Ile-Gln
Host
E Coli
Form/Format
The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.
White lyophilized (freeze-dried) powder.
Purity/Purification
Greater than 98.0% as determined by: (a) Gel filtration analysis. (b) Analysis by SDS-PAGE.
Preparation and Storage
Lyophilized Leptin Antagonist Triple Mutant Human Recombinant although stable at room temperature for several weeks, should be stored desiccated below -18 degree C. Upon reconstitution at > 0.1 mg/ml and up to 2 mM and filter sterilization Leptin mutant can be stored at 4 degree C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Solubility
It is recommended to reconstitute the lyophilized Leptin Antagonist Triple Mutant Human Recombinant in sterile 0.4% NaHCO3adjusted to pH 8-9, not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Protein Content
Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.88 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Biological Activity
MyBioSource's Leptin triple antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. It also inhibits various leptin effects in several in vitro bioassays.

Product Categories/Family for Leptin Triple Antagonist active protein

Cytokines and Growth Factors; Growth Factors; Leptin

Other Notes

Small volumes of Leptin Triple Antagonist active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Leptin Triple Antagonist purchase

MBS142229 is an active protein targeting Leptin Triple Antagonist. Also known as Recombinant Human Leptin Triple Antagonist. Host: E Coli.
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