Mouse mIL 7 Active Protein – Cat# MBS142249

Interleukin-7 (mIL 7) Mouse Active Protein

Name: mIL 7 Active Protein; Interleukin-7; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS142249
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS142249

Description: Interleukin-7 Mouse Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15.0 kDa. The IL-7 is purified by proprietary chromatographic techniques.Introduction:… More details

$250 / 0.002 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Interleukin-7 (mIL 7), Active Protein

Full Product Name

Recombinant Mouse Interleukin-7

Product Gene Name

mIL 7 active protein

Product Synonym Names

IL 7 Mouse; Interleukin-7 Mouse Recombinant; Lymphopoietin 1 (LP-1); pre-B cell factor; IL-7

Sequence

ECHIKDKEGK AYESVLMISI DELDKMTGTD SNCPNNEPNF FRKHVCDDTK EAAFLNRAAR KLKQFLKMNI SEEFNVHLLT VSQGTQTLVN CTSKEEKNVK EQKKNDACFL KRLLREIKTC WNKILKGSI

Host

E Coli

Form/Format

Filtered (0.2 um) and lyophilized from a concentrated (1mg/ml) solution in 1xPBS, pH7.4.
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity/Purification

Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Preparation and Storage

Lyophilized Interleukin-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution IL7 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Molecular Weight

17,727 Da

3D Structure

ModBase 3D Structure for P10168

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility

It is recommended to reconstitute the lyophilized Interleukin 7 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.

Protein Content

Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.48 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-7 as a Reference Standard.

Biological Activity

The ED50 as determined by the dose-dependant stimulation of murine 2E8 cells is < 0.2ng/ml, corresponding to a Specific Activity of 5,000,000 units/mg.

Product Categories/Family for mIL 7 active protein

Cytokines and Growth Factors; Cytokines; Interleukin

Other Notes

Small volumes of mIL 7 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for mIL 7 purchase

MBS142249 is an active protein targeting mIL 7. Also known as Recombinant Mouse Interleukin-7. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for mIL 7. It may not necessarily be applicable to this product.

NCBI GI #

6680433

NCBI GeneID

16196

NCBI Accession #

NP_032397.1

UniProt Primary Accession #

P10168

UniProt Related Accession #

P10168

GenBank Nucleotide #

NM_008371.4

NCBI Official Full Name

interleukin-7

NCBI Official Synonym Full Names

interleukin 7

NCBI Official Symbol

Il7

NCBI Official Synonym Symbols

Il-7; hlb368; A630026I06Rik

UniProt Protein Name

Interleukin-7

Protein Family

Interleukin-7 receptor

UniProt Gene Name

Il7

UniProt Synonym Gene Names

Il-7; IL-7

UniProt Entry Name

IL7_MOUSE

NCBI Protein Information

interleukin-7

NCBI Summary for mIL 7

The protein encoded by this gene is a hematopoietic growth factor important for B and T cell development. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

UniProt Comments for mIL 7

IL7: Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. Belongs to the IL-7/IL-9 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Secreted, signal peptide; Cell development/differentiation; Cytokine; Secreted; Apoptosis

Cellular Component: extracellular space; extracellular region

Molecular Function: interleukin-7 receptor binding; growth factor activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; cytokine activity

Biological Process: organ morphogenesis; T cell lineage commitment; positive regulation of T cell differentiation; cell-cell signaling; regulation of gene expression; homeostasis of number of cells within a tissue; negative regulation of catalytic activity; positive regulation of B cell proliferation; immune response; positive regulation of organ growth; humoral immune response; bone resorption

Research Articles on mIL 7

1. OX40 and IL-7 play synergistic, but distinct roles in the homeostatic proliferation of CD4(+) effector memory T cells

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with mIL 7 active protein

Products by Pathway	Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokines And Inflammatory Response (BioCarta) Pathway antibodies	Cytokines And Inflammatory Response (BioCarta) Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Interleukin-7 Signaling Pathway antibodies	Interleukin-7 Signaling Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram

Diseases associated with mIL 7 active protein

Disease Name	Pubmed Publications
Inflammation Antibodies	>27 publications with mIL 7 and Inflammation
Necrosis Antibodies	>21 publications with mIL 7 and Necrosis
Multiple Sclerosis Antibodies	>19 publications with mIL 7 and Multiple Sclerosis
Lymphopenia Antibodies	>17 publications with mIL 7 and Lymphopenia
Neoplasms, Experimental Antibodies	>15 publications with mIL 7 and Neoplasms, Experimental
Death Antibodies	>14 publications with mIL 7 and Death
Carcinoma Antibodies	>11 publications with mIL 7 and Carcinoma
Cell Transformation, Neoplastic Antibodies	>7 publications with mIL 7 and Cell Transformation, Neoplastic
Sepsis Antibodies	>6 publications with mIL 7 and Sepsis
Hypersensitivity Antibodies	>6 publications with mIL 7 and Hypersensitivity

Organs/Tissues associated with mIL 7 active protein

Organ/Tissue Name	Pubmed Publications
Other Antibodies	>42 publications with mIL 7 and Other
Thymus Antibodies	>40 publications with mIL 7 and Thymus
Spleen Antibodies	>12 publications with mIL 7 and Spleen
Lung Antibodies	>10 publications with mIL 7 and Lung
Liver Antibodies	>10 publications with mIL 7 and Liver
Embryonic Tissue Antibodies	>4 publications with mIL 7 and Embryonic Tissue
Ovary Antibodies	>3 publications with mIL 7 and Ovary
Mammary Gland Antibodies	>2 publications with mIL 7 and Mammary Gland