Mouse mIL 20 Active Protein – Cat# MBS142256

Interleukin-20 (mIL 20) Mouse Active Protein

Name: mIL 20 Active Protein; Interleukin-20; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS142256
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS142256

Description: Interleukin-20 Mouse Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 152 amino acids and having a total molecular mass of 17,584 Dalton. The IL-20 is purified by proprietary chromatographic… More details

$250 / 0.002 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Interleukin-20 (mIL 20), Active Protein

Full Product Name

Recombinant Mouse Interleukin-20

Product Gene Name

mIL 20 active protein

Product Synonym Names

IL 20 Mouse; Interleukin-20 Mouse Recombinant; IL10D; ZCYTO10; IL-20; MGC96907; Four alpha helix cytokine Zcyto10

Sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Ly s-Thr-Leu

Host

E Coli

Form/Format

Lyophilized from a concentrated (1mg/ml) solution containing no additives.
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity/Purification

Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Preparation and Storage

Lyophilized Interleukin-20 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution IL20 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Molecular Weight

20,098 Da

3D Structure

ModBase 3D Structure for Q9JKV9

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility

It is recommended to reconstitute the lyophilized Interleukin-20 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.

Protein Content

Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.552 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-20 as a Reference Standard.

Biological Activity

The biological activity was determined by measuring the dose dependent activation of STAT3 in human epidermal keratinocytes (HEK2a cells). Significant STAT3 activation is observed with >100ng/mL of Interleukin-20 recombinant mouse, corresponding to a specific activity of 10,000 units/mg of Interleukin-20 recombinant mouse.

Product Categories/Family for mIL 20 active protein

Cytokines and Growth Factors; Cytokines; Interleukin

Other Notes

Small volumes of mIL 20 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for mIL 20 purchase

MBS142256 is an active protein targeting mIL 20. Also known as Recombinant Mouse Interleukin-20. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for mIL 20. It may not necessarily be applicable to this product.

NCBI GI #

10946716

NCBI GeneID

58181

NCBI Accession #

NP_067355.1

UniProt Primary Accession #

Q9JKV9

UniProt Related Accession #

Q9JKV9

GenBank Nucleotide #

NM_021380.1

NCBI Official Full Name

interleukin-20

NCBI Official Synonym Full Names

interleukin 20

NCBI Official Symbol

Il20

NCBI Official Synonym Symbols

Zcyto10

UniProt Protein Name

Interleukin-20

UniProt Synonym Protein Names

Cytokine Zcyto10

Protein Family

Interleukin-20 receptor

UniProt Gene Name

Il20

UniProt Synonym Gene Names

Zcyto10; IL-20

UniProt Entry Name

IL20_MOUSE

NCBI Protein Information

interleukin-20; IL-20; cytokine Zcyto10; four alpha helix cytokine ZCYTO10; four helix bundle cytokine 10

UniProt Comments for mIL 20

IL20: Cytokine that may be involved in epidermal function and psoriasis. Acts through STAT3. Belongs to the IL-10 family.

Protein type: Secreted, signal peptide; Secreted; Cytokine

Cellular Component: extracellular space; cell; extracellular region; intracellular

Molecular Function: interleukin-20 receptor binding; cytokine activity; interleukin-22 receptor binding; receptor binding

Biological Process: positive regulation of osteoclast differentiation; positive regulation of JAK-STAT cascade; hemopoiesis; immune response; inflammatory response; STAT protein nuclear translocation

Research Articles on mIL 20

1. our results suggest that IL-20 plays pivotal roles in the tumor progression of breast cancer. IL-20 expression in breast cancer tissue is associated with a poor clinical outcome.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with mIL 20 active protein

Products by Pathway	Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram

Diseases associated with mIL 20 active protein

Disease Name	Pubmed Publications
Immune System Diseases Antibodies	>5 publications with mIL 20 and Immune System Diseases
Inflammation Antibodies	>4 publications with mIL 20 and Inflammation
Autoimmune Diseases Antibodies	>3 publications with mIL 20 and Autoimmune Diseases
Lung Diseases Antibodies	>3 publications with mIL 20 and Lung Diseases
Necrosis Antibodies	>2 publications with mIL 20 and Necrosis
Fibrosis Antibodies	>2 publications with mIL 20 and Fibrosis
Nervous System Diseases Antibodies	>1 publications with mIL 20 and Nervous System Diseases
Fatty Liver Antibodies	>1 publications with mIL 20 and Fatty Liver
Pulmonary Fibrosis Antibodies	>1 publications with mIL 20 and Pulmonary Fibrosis
Brain Diseases Antibodies	>1 publications with mIL 20 and Brain Diseases

Organs/Tissues associated with mIL 20 active protein

Organ/Tissue Name	Pubmed Publications
Eye Antibodies	>1 publications with mIL 20 and Eye