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Interleukin-2 (rIL 2) Rat Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS142259

Description: Interleukin-2 Rat Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 134 amino acids and having a molecular mass of 17kDa. The IL-2 is purified by proprietary chromatographic techniques.Introduction: IL2 is… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Interleukin-2 (rIL 2), Active Protein
Full Product Name
Recombinant Rat Interleukin-2
Product Gene Name
rIL 2 active protein
Product Synonym Names
IL 2 Rat; Interleukin-2 Rat Recombinant; T-cell growth factor (TCGF); Aldesleukin; Lymphokine; IL-2
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pr o-Thr-Ser
Host
E Coli
Form/Format
The protein (1mg/ml) was lyophilized without any additives.
Purity/Purification
Greater than 95.0% as determined by SDS-PAGE.
Preparation and Storage
Lyophilized Interleukin-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18° C. Upon reconstitution IL2 should be stored at 4° C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Molecular Weight
17,633 Da
3D Structure
ModBase 3D Structure for P17108
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Physical Appearance
Sterile Filtered White Lyophilized (freeze-dried) powder.
Solubility
It is recommended to reconstitute the lyophilized Interleukin 2 in sterile 100mM AcOH not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Protein Content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.535 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IL-2 as a Reference Standard.
Biological Activity
The ED50 as determined by the dose-dependant stimulation of murine CTLL-2 cells is 0.1-0.4 ng/ml corresponding to a specific activity of 2,500,000-10,000,000 units/mg.

Product Categories/Family for rIL 2 active protein

Cytokines and Growth Factors; Cytokines; Interleukin

Other Notes

Small volumes of rIL 2 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for rIL 2 purchase

MBS142259 is an active protein targeting rIL 2. Also known as Recombinant Rat Interleukin-2. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for rIL 2. It may not necessarily be applicable to this product.

NCBI GI #
16758692
NCBI GeneID
116562
NCBI Accession #
NP_446288.1
UniProt Primary Accession #
P17108
UniProt Related Accession #
P17108
GenBank Nucleotide #
NM_053836.1
NCBI Official Full Name
interleukin-2
NCBI Official Synonym Full Names
interleukin 2
NCBI Official Symbol
Il2
UniProt Protein Name
Interleukin-2
UniProt Synonym Protein Names
T-cell growth factor; TCGF
Protein Family
Interleukin-2 receptor
UniProt Gene Name
Il2
UniProt Synonym Gene Names
Il-2; IL-2; TCGF
UniProt Entry Name
IL2_RAT
NCBI Protein Information
interleukin-2; IL-2; T-cell growth factor; TCGF

NCBI Summary for rIL 2

cytokine produced by T-cells in response to antigen or mitogen stimulation [RGD, Feb 2006]

UniProt Comments for rIL 2

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.

Research Articles on rIL 2

1. Data indicate that C-rel binding on the interleukin-2 (IL-2) promoter region declined in the aged CD4+T cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with rIL 2 active protein

 Reference Product  PubMed Publications
 Il4 active protein  >382 publications with rIL 2 and Il4
 Il2ra active protein  >115 publications with rIL 2 and Il2ra
 Il5 active protein  >101 publications with rIL 2 and Il5
 Foxp3 active protein  >98 publications with rIL 2 and Foxp3
 Il7 active protein  >42 publications with rIL 2 and Il7
 Jak3 active protein  >13 publications with rIL 2 and Jak3
 Jak1 active protein  >12 publications with rIL 2 and Jak1
 Il2rg active protein  >12 publications with rIL 2 and Il2rg
 Csf2 active protein  >10 publications with rIL 2 and Csf2
 Syk active protein  >5 publications with rIL 2 and Syk

Pathways associated with rIL 2 active protein

 Products by Pathway  Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokines And Inflammatory Response (BioCarta) Pathway antibodies  Cytokines And Inflammatory Response (BioCarta) Pathway Diagram

Diseases associated with rIL 2 active protein

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >314 publications with rIL 2 and Necrosis
 Leukemia Antibodies  >229 publications with rIL 2 and Leukemia
 Inflammation Antibodies  >200 publications with rIL 2 and Inflammation
 Melanoma Antibodies  >143 publications with rIL 2 and Melanoma
 Neoplasms, Experimental Antibodies  >142 publications with rIL 2 and Neoplasms, Experimental
 Kidney Diseases Antibodies  >109 publications with rIL 2 and Kidney Diseases
 Liver Diseases Antibodies  >97 publications with rIL 2 and Liver Diseases
 HIV Infections Antibodies  >73 publications with rIL 2 and HIV Infections
 Kidney Neoplasms Antibodies  >71 publications with rIL 2 and Kidney Neoplasms
 Lung Neoplasms Antibodies  >62 publications with rIL 2 and Lung Neoplasms
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