Pseudomonas Syringae Pv. Tomato avrRpt2 Recombinant Protein – Cat# MBS1422609

Cysteine Protease Avirulence Protein Avrrpt2 (avrRpt2) Pseudomonas Syringae Pv. Tomato Recombinant Protein

Name: avrRpt2 Recombinant Protein; Pseudomonas Syringae Pv. Tomato; MyBioSource
Brand: MyBioSource
SKU: MBS1422609
Price: 825 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1422609

MBS1422609 is a recombinant protein targeting avrRpt2. Also known as Recombinant Pseudomonas syringae pv. tomato Cysteine protease avirulence protein AvrRpt2 (avrRpt2). Host: E Coli or Yeast or Baculovirus or Mammalian Cell. More details

$825 / 0.02 mg (E-Coli) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Cysteine protease avirulence protein AvrRpt2 (avrRpt2), Recombinant Protein

Full Product Name

Recombinant Pseudomonas syringae pv. tomato Cysteine protease avirulence protein AvrRpt2 (avrRpt2)

Product Gene Name

avrRpt2 recombinant protein

Product Synonym Gene Name

avrRpt2

Sequence

GWFKKKSSKH ETGGSSANAD SSSVASDSTE KPLFRLTHVP YVSQGNERMG CWYACARMVG HSVEAGPRLG LPELYEGREG PAGLQDFSDV ERFIHNEGLT RVDLPDNERF THEELGALLY KHGPIIFGWK TPNDSWHMSV LTGVDKETSS ITFHDPRQGP DLAMPLDYFN QRLAWQVPHA MLYR

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Form/Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Purity/Purification

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Preparation and Storage

Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Molecular Weight

28,158 Da

Sequence Positions

72-255, Full length protein

Tag Information

This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.

Sterility

Sterile filter available upon request.

Endotoxin

Low endotoxin available upon request.

3D Structure

ModBase 3D Structure for Q6LAD6

Species

Pseudomonas syringae pv. tomato

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of avrRpt2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for avrRpt2. It may not necessarily be applicable to this product.

NCBI GI #

75368208

NCBI Accession #

Q6LAD6.1

UniProt Primary Accession #

Q6LAD6

UniProt Secondary Accession #

Q05324; Q4LBF4

NCBI Official Full Name

Cysteine protease avirulence protein AvrRpt2

UniProt Protein Name

Cysteine protease avirulence protein AvrRpt2

Protein Family

Cysteine protease avirulence protein

UniProt Gene Name

avrRpt2

UniProt Synonym Gene Names

Avirulence protein AvrRpt2

UniProt Comments for avrRpt2

Effector protein involved in gene-for-gene resistance in plants expressing RPS2. Its thiol protease activity is required for the degradation of plant cell RIN4 and consequent activation of RPS2 during bacterial infection. The activation of RPS2 is sufficient for the induction of hypersensitive response (HR) and plant resistance. Cleavage of RIN4 by AvrRpt2 also interferes with RPM1-mediated resistance activated by either AvrRpm1 or AvrB. Contributes to virulence in plants lacking the resistance protein RPS2 promoting pathogen growth and disease symptoms. Inhibits PAMP (pathogen-associated molecular patterns)-induced signaling compromising the host's basal defense system. Blocks plant callose deposition, flg22 (a peptide corresponding to the most conserved domain of flagellin) induced accumulation of PR-1, PR-2 and PR-5 and activation of GST6 transcription. The mechanism of virulence is unknown, but this activity is independent of ethylene and salicylic acid response pathways and independent of RIN4 disappearance.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.