• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Interferon-Inducible Double Stranded RNA-Dependent Protein Kinase Activator A (PRKRA) Bovine Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1422845

Activates EIF2AK2PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated… More details

$385 / 0.02 mg (E-Coli) +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Interferon-inducible double stranded RNA-dependent protein kinase activator A (PRKRA), Recombinant Protein
Full Product Name
Recombinant Bovine Interferon-inducible double stranded RNA-dependent protein kinase activator A (PRKRA)
Product Gene Name
PRKRA recombinant protein
Product Synonym Gene Name
PRKRA
Sequence
MSQSRHRAAA PPMEREDSGT FSLGKMITAK PGKTPIQVLH EYGMKTKNIP VYECERSDVQ IHVPTFTFRV TVGDITCTGE GTSKKLAKHR AAEAAINILK ANASICFAVP DPLMPDPSKQ PKNQLNPIGS LQELAIHHGW RLPEYTLSQE GGPAHKREYT TICRLESFME TGKGASKKQA KRNAAEKFLA KFSNISPENH ISLTNMVGHS LGCTWHSLRN SPGEKINLLK RSLLSIPNTD YIQLLSEIAK EQGFNITYLD IEELSANGQY QCLAELSTSP ITVCHGSGIS CSSAQSDAAH NALQYLKIIA ERK
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
Molecular Weight
39.4 kDa
Sequence Positions
1-313aa; Full Length
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
3D Structure
ModBase 3D Structure for Q2HJ92
Species
Bos taurus (Bovine)
Production Note
Special Offer: The E Coli host-expressed protein is manufactured from a stock plasmid containing the protein gene. E Colihost-expressed protein is stocked in different unit sizes ranging from as small as 10 ug to as large as 1 mg. Bulk inventory is also available. The E Coli host-expressed protein has been ordered over and over again by researchers and has stood the test of time as both a robust protein and important target for the research community. It is part of our new program to make our most popular protein targets and corresponding hosts available in expanded unit sizes and with a quick processing time. Select E Coli host-expressed protein for the fastest delivery among all hosts. Please contact our technical support team or email to support@mybiosource.com for more details.

Product Categories/Family for PRKRA recombinant protein

Epigenetics and Nuclear Signaling
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

SDS-Page of PRKRA recombinant protein

Bovine PRKRA Recombinant Protein – Cat# MBS1422845

Other Notes

Small volumes of PRKRA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PRKRA purchase

MBS1422845 is a recombinant protein targeting PRKRA. Also known as Recombinant Bovine Interferon-inducible double stranded RNA-dependent protein kinase activator A (PRKRA). Host: E Coli or Yeast or Baculovirus or Mammalian Cell.

Additional Details

NCBI/Uniprot data below describe general gene information for PRKRA. It may not necessarily be applicable to this product.

NCBI GI #
114052769
NCBI GeneID
282875
NCBI Accession #
NP_001039335.1
UniProt Primary Accession #
Q2HJ92
UniProt Related Accession #
Q2HJ92
GenBank Nucleotide #
NM_001045870.1
NCBI Official Full Name
interferon-inducible double-stranded RNA-dependent protein kinase activator A
NCBI Official Synonym Full Names
protein activator of interferon induced protein kinase EIF2AK2
NCBI Official Symbol
PRKRA
NCBI Official Synonym Symbols
PACT
UniProt Protein Name
Interferon-inducible double-stranded RNA-dependent protein kinase activator A
UniProt Synonym Protein Names
Protein activator of the interferon-induced protein kinase; Protein kinase, interferon-inducible double-stranded RNA-dependent activator
Protein Family
Interferon-inducible double-stranded RNA-dependent protein kinase activator
UniProt Gene Name
PRKRA
NCBI Protein Information
interferon-inducible double-stranded RNA-dependent protein kinase activator A

UniProt Comments for PRKRA

Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner ().
Show more

Research Articles on PRKRA

1. Tumour necrosis factor alpha up-regulates protein kinase R (PKR)-activating protein (PACT, PRKRA) and increases phosphorylation of PKR and eukaryotic initiation factor 2-alpha in articular chondrocytes.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PRKRA recombinant protein

 Reference Product  PubMed Publications
 DICER1 recombinant protein  >8 publications with PRKRA and DICER1
 EIF2C2 recombinant protein  >6 publications with PRKRA and EIF2C2
 TARBP2 recombinant protein  >4 publications with PRKRA and TARBP2
 ATP7B recombinant protein  >1 publications with PRKRA and ATP7B
 PPP1CA recombinant protein  >1 publications with PRKRA and PPP1CA
 RNASEN recombinant protein  >1 publications with PRKRA and RNASEN

Pathways associated with PRKRA recombinant protein

 Products by Pathway  Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 MicroRNA (miRNA) Biogenesis Pathway antibodies  MicroRNA (miRNA) Biogenesis Pathway Diagram
 Regulatory RNA Pathways antibodies  Regulatory RNA Pathways Diagram
 Small Interfering RNA (siRNA) Biogenesis Pathway antibodies  Small Interfering RNA (siRNA) Biogenesis Pathway Diagram

Diseases associated with PRKRA recombinant protein

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >12 publications with PRKRA and Nervous System Diseases
 Dystonia Antibodies  >11 publications with PRKRA and Dystonia
 Brain Diseases Antibodies  >8 publications with PRKRA and Brain Diseases
 Necrosis Antibodies  >1 publications with PRKRA and Necrosis
 Seizures Antibodies  >1 publications with PRKRA and Seizures
 Precancerous Conditions Antibodies  >1 publications with PRKRA and Precancerous Conditions
 Hyperplasia Antibodies  >1 publications with PRKRA and Hyperplasia
 Disease Models, Animal Antibodies  >1 publications with PRKRA and Disease Models, Animal

Organs/Tissues associated with PRKRA recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >8 publications with PRKRA and Brain
 Muscle Antibodies  >2 publications with PRKRA and Muscle
 Intestine Antibodies  >1 publications with PRKRA and Intestine
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000