Human MSTN Active Protein – Cat# MBS142294

Myostatin (MSTN) Human Active Protein

Name: MSTN Active Protein; Myostatin; Human; MyBioSource
Brand: MyBioSource
SKU: MBS142294
Price: 250 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS142294

Description: Myostatin Human Recombinant produced in E Coli is a homodimer, non-glycosylated polypeptide chain containing 2 x 109 amino acids and having a total molecular mass of 24814 Dalton. The GDF-8 is purified by proprietary chromatographic… More details

$250 / 0.002 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Myostatin (MSTN), Active Protein

Full Product Name

Recombinant Human Myostatin

Product Gene Name

MSTN active protein

Product Synonym Names

Myostatin Human; Myostatin Human Recombinant; GDF-8; MSTN; Growth Differentiation Factor 8; MSTN Muscle Hypertrophy; Myostatin

Sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Asp-Phe-Gl y-Leu-Asp

Host

E Coli

Form/Format

Lyophilized from a concentrated (1mg/ml) solution containing no additives.
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity/Purification

Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Preparation and Storage

Lyophilized Myostatin although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Myostatin should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Molecular Weight

42,750 Da

OMIM

601788

3D Structure

ModBase 3D Structure for O14793

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility

It is recommended to reconstitute the lyophilized Myostatin in sterile 20mM HCl at 0.1 mg/ml, which can then be further diluted to other aqueous solutions.

Protein Content

Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 1.55 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of Myostatin as a Reference Standard.

Biological Activity

The ED50 as determined by the inhibition of the proliferation of MPC-11 cells is < 20ng/ml, corresponding to a Specific Activity of 50,000units/mg.

Product Categories/Family for MSTN active protein

Cytokines and Growth Factors; Growth Factors; Myostatin

Other Notes

Small volumes of MSTN active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MSTN purchase

MBS142294 is an active protein targeting MSTN. Also known as Recombinant Human Myostatin. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for MSTN. It may not necessarily be applicable to this product.

NCBI GI #

4885259

NCBI GeneID

2660

NCBI Accession #

NP_005250.1

UniProt Primary Accession #

O14793

UniProt Secondary Accession #

Q6B0H2; A1C2J7; A1C2K0

UniProt Related Accession #

O14793

GenBank Nucleotide #

NM_005259.2

NCBI Official Full Name

growth/differentiation factor 8

NCBI Official Synonym Full Names

myostatin

NCBI Official Symbol

MSTN

NCBI Official Synonym Symbols

GDF8; MSLHP

UniProt Protein Name

Growth/differentiation factor 8

UniProt Synonym Protein Names

Myostatin

Protein Family

Growth/differentiation factor

UniProt Gene Name

MSTN

UniProt Synonym Gene Names

GDF8; GDF-8

UniProt Entry Name

GDF8_HUMAN

NCBI Protein Information

growth/differentiation factor 8; GDF-8; growth differentiation factor 8

NCBI Summary for MSTN

The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. This gene is thought to encode a secreted protein which negatively regulates skeletal muscle growth. [provided by RefSeq, Jul 2008]

UniProt Comments for MSTN

MSTN: Acts specifically as a negative regulator of skeletal muscle growth. Defects in MSTN are the cause of muscle hypertrophy (MSLHP). MSLHP is a condition characterized by increased muscle bulk and strength. Affected individuals are exceptionally strong. Belongs to the TGF-beta family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 2q32.2

Cellular Component: extracellular space; cytoplasm

Molecular Function: heparin binding; identical protein binding; protein binding; growth factor activity; cytokine activity; transforming growth factor beta receptor binding; receptor binding

Biological Process: response to gravity; response to muscle activity; muscle development; positive regulation of transcription, DNA-dependent; skeletal muscle regeneration; response to testosterone stimulus; ovulation cycle process; regulation of apoptosis; negative regulation of muscle hypertrophy; skeletal muscle atrophy; response to ethanol; response to heat; response to estrogen stimulus; transforming growth factor beta receptor signaling pathway; regulation of MAPKKK cascade; negative regulation of skeletal muscle growth; response to electrical stimulus; cell development

Disease: Muscle Hypertrophy

Research Articles on MSTN

1. role of myostatin in cardiovascular disease and cachexia

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MSTN active protein

Reference Product	PubMed Publications
MYOD1 active protein	>25 publications with MSTN and MYOD1
FSTL3 active protein	>22 publications with MSTN and FSTL3
IGF1 active protein	>16 publications with MSTN and IGF1
FOXO1 active protein	>12 publications with MSTN and FOXO1
DCN active protein	>8 publications with MSTN and DCN
SMAD7 active protein	>5 publications with MSTN and SMAD7
WFIKKN1 active protein	>2 publications with MSTN and WFIKKN1
WFIKKN2 active protein	>2 publications with MSTN and WFIKKN2
INS active protein	>1 publications with MSTN and INS

Pathways associated with MSTN active protein

Products by Pathway	Pathway Diagram
Hypertrophy Model Pathway antibodies	Hypertrophy Model Pathway Diagram

Diseases associated with MSTN active protein

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>154 publications with MSTN and Nervous System Diseases
Hypertrophy Antibodies	>151 publications with MSTN and Hypertrophy
Atrophy Antibodies	>125 publications with MSTN and Atrophy
Muscular Atrophy Antibodies	>90 publications with MSTN and Muscular Atrophy
Weight Loss Antibodies	>47 publications with MSTN and Weight Loss
Hyperplasia Antibodies	>36 publications with MSTN and Hyperplasia
Disease Models, Animal Antibodies	>34 publications with MSTN and Disease Models, Animal
Insulin Resistance Antibodies	>34 publications with MSTN and Insulin Resistance
Inflammation Antibodies	>19 publications with MSTN and Inflammation
Kidney Diseases Antibodies	>19 publications with MSTN and Kidney Diseases

Organs/Tissues associated with MSTN active protein

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>723 publications with MSTN and Muscle
Connective Tissue Antibodies	>54 publications with MSTN and Connective Tissue
Heart Antibodies	>53 publications with MSTN and Heart
Embryonic Tissue Antibodies	>43 publications with MSTN and Embryonic Tissue
Brain Antibodies	>35 publications with MSTN and Brain
Eye Antibodies	>8 publications with MSTN and Eye
Placenta Antibodies	>7 publications with MSTN and Placenta
Lung Antibodies	>6 publications with MSTN and Lung