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Tissue Plasminogen Activator (tPA) Human Active Protein

For Research Use Only. Not for use in diagnostic procedures. Popular Catalog #: MBS142404

This human tPA (Tissue Plasminogen Activator) active protein, produced in CHO cells, is a single glycosylated polypeptide chain of 527 amino acids (59 kDa) that functions as a serine protease converting plasminogen to plasmin. Tissue Plasminogen Activator… More details

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Product Overview

Online Datasheet in PDF
Product Name
Tissue Plasminogen Activator (tPA), Active Protein
Full Product Name
Recombinant Human Tissue Plasminogen Activator
Product Gene Name
tPA active protein
Product Synonym Names
Tissue-type plasminogen activator, EC 3.4.21.68, tPA, t-PA, t-plasminogen activator, TPA, T-PA, DKFZp686I03148.
Form/Format
Each mg of t-PA contains 1.7 gr L-arginine, 0.5 gr phosphoric acid and 4 mg tween 80.
Purity/Purification
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Preparation and Storage
Lyophilized t-PA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution tPA should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
OMIM
173370
3D Structure
ModBase 3D Structure for P00750
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Source
Chinese Hamster Ovary Cells (CHO)
Enzymatic Activity
580,000 IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Solubility
It is recommended to reconstitute the lyophilized t-PA in sterile 18MΩ-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.

Product Categories/Family for tPA active protein

Enzymes

Other Notes

Small volumes of tPA active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for tPA purchase

Human tissue plasminogen activator (tPA) is also known as alteplase, TPA, T-PA, t-PA, PLAT and tissue-type plasminogen activator.

tPA is secreted serine protease (EC 3.4.21.68), naturally occurring in endothelial cells of blood vessels; it converts proenzyme plasminogen to its active form, plasmin, enzyme responsible for breakdown of blood clots ; increased activity of tPA may cause hyperfibrinolysis and excessive bleeding including intracranial haemorrhage, and reduced activity may result in thrombosis or embolism due to hypofibrinolysis.

tPA is recognized for its role in thrombolysis (blood clots breakdown) and administered at ischemic (ischaemic) conditions such as acute ischemic (ischaemic) stroke. Due to tPA specific functions in blood clotting system, it’s used clinically to treat thrombolytic or embolic stroke, acute myocardial infarction, pulmonary embolism, and its use is contradicted in cases when bleeding is expected such as head trauma or hemorrhagic stroke.

tPA is recombinant protein produced in CHO cells as single-chain glycosylated polypeptide containing 527 amino acids and with molecular mass of 59kDA, and is purified by proprietary chromatographic techniques; its purity is greater than 98% (by RP-HPLC and SDS-PAGE) and activity is 580,000IU/mg.

It’s provided as lyophilized preparation and is stable for up to 3 weeks at ambient temperature; recommended storage is below -18°C, dessicated.

Additional Details

NCBI/Uniprot data below describe general gene information for tPA. It may not necessarily be applicable to this product.

NCBI GI #
4505861
NCBI GeneID
5327
NCBI Accession #
NP_000921.1
UniProt Primary Accession #
P00750
UniProt Secondary Accession #
Q15103; Q503B0; Q6PJA5; Q7Z7N2; Q86YK8; Q9BU99; Q9BZW1; A8K022; B2R8E8
UniProt Related Accession #
P00750
GenBank Nucleotide #
NM_000930.3
NCBI Official Full Name
tissue-type plasminogen activator isoform 1 preproprotein
NCBI Official Synonym Full Names
plasminogen activator, tissue
NCBI Official Symbol
PLAT
NCBI Official Synonym Symbols
TPA; T-PA
UniProt Protein Name
Tissue-type plasminogen activator
UniProt Synonym Protein Names
INN: Alteplase; INN: ReteplaseCleaved into the following 2 chains:Tissue-type plasminogen activator chain A; Tissue-type plasminogen activator chain B
Protein Family
Taurine--pyruvate aminotransferase
UniProt Gene Name
PLAT
UniProt Synonym Gene Names
t-PA; t-plasminogen activator; tPA
UniProt Entry Name
TPA_HUMAN
NCBI Protein Information
tissue-type plasminogen activator; alteplase; plasminogen activator, tissue type; reteplase; t-plasminogen activator; tissue plasminogen activator (t-PA)

NCBI Summary for tPA

This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
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UniProt Comments for tPA

PLAT: Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Plays a direct role in facilitating neuronal migration. Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism. Belongs to the peptidase S1 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal; Vesicle; Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; EC 3.4.21.68; Protease

Chromosomal Location of Human Ortholog: 8p12

Cellular Component: extracellular matrix; extracellular space; cell surface; apical part of cell; cytoplasm; extracellular region; synapse; secretory granule

Molecular Function: protein binding; serine-type endopeptidase activity

Biological Process: negative regulation of proteolysis; synaptic transmission, glutamatergic; response to peptide hormone stimulus; fibrinolysis; response to cAMP; smooth muscle cell migration; response to hypoxia; regulation of synaptic plasticity; protein modification process; platelet-derived growth factor receptor signaling pathway; proteolysis; blood coagulation; transmembrane transport; plasminogen activation

Disease: Thrombophilia, Familial, Due To Decreased Release Of Tissue Plasminogen Activator
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Research Articles on tPA

1. Report tPA with a cutoff of 8.5 ng/mL has a high sensitivity and negative predictive value for exclusion of pulmonary embolism.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with tPA active protein

 Products by Pathway  Pathway Diagram
 Blood Clotting Cascade Pathway antibodies  Blood Clotting Cascade Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Dissolution Of Fibrin Clot Pathway antibodies  Dissolution Of Fibrin Clot Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Orphan Transporters Pathway antibodies  Orphan Transporters Pathway Diagram
 Selenium Pathway antibodies  Selenium Pathway Diagram
 Senescence And Autophagy Pathway antibodies  Senescence And Autophagy Pathway Diagram

Diseases associated with tPA active protein

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >38 publications with tPA and Inflammation
 Arterial Occlusive Diseases Antibodies  >25 publications with tPA and Arterial Occlusive Diseases
 Atherosclerosis Antibodies  >25 publications with tPA and Atherosclerosis
 Thrombosis Antibodies  >20 publications with tPA and Thrombosis
 Cerebral Infarction Antibodies  >19 publications with tPA and Cerebral Infarction
 Stroke Antibodies  >18 publications with tPA and Stroke
 Necrosis Antibodies  >17 publications with tPA and Necrosis
 Myocardial Infarction Antibodies  >14 publications with tPA and Myocardial Infarction
 Hypertension Antibodies  >14 publications with tPA and Hypertension
 Embolism and Thrombosis Antibodies  >12 publications with tPA and Embolism and Thrombosis

Organs/Tissues associated with tPA active protein

 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >54 publications with tPA and Vascular
 Liver Antibodies  >49 publications with tPA and Liver
 Brain Antibodies  >45 publications with tPA and Brain
 Heart Antibodies  >41 publications with tPA and Heart
 Lung Antibodies  >35 publications with tPA and Lung
 Muscle Antibodies  >29 publications with tPA and Muscle
 Bone Antibodies  >20 publications with tPA and Bone
 Intestine Antibodies  >18 publications with tPA and Intestine
 Embryonic Tissue Antibodies  >16 publications with tPA and Embryonic Tissue
 Kidney Antibodies  >15 publications with tPA and Kidney
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