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Taq DNA Polymerase (polA) Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS142449

Description: Taq DNA Polymerase(a) is a thermostable enzyme of approximately 95 kDa isolated from Thermus aquaticus. This unmodified enzyme replicates DNA at 74°C and exhibits a half-life of 40 minutes at 95°C. The enzyme catalyzes the polymerization of… More details

$250 / 1000 Units +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Taq DNA Polymerase (polA), Recombinant Protein
Full Product Name
Recombinant Taq DNA Polymerase
Product Gene Name
polA recombinant protein
Product Synonym Names
TaqDNA; Taq DNA Polymerase Recombinant; DNA polymerase I thermostable; EC 2.7.7.7; Taq polymerase 1; TaqDNA
Host
Recombinant E Coli contains Thermus aquaticus polymerase gene
Form/Format
Tag DNA Polymerase solution (10,000 IU/ 500 uL) in 20 mM Tris-HCI, pH 8.0, 100 mM KCI 0.1 mM EDTA, 1 mM DTT, 50% Glycerol,0.5% NP40, 0.5% Tween 20.
Purity/Purification
Greater than 95.0% as determined by SDS-PAGE
Preparation and Storage
Stable for 5 days at 10°C, for longer period of time store at -20°C.
3D Structure
ModBase 3D Structure for P19821
Unit Definition
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. The reaction conditions are: 50mM Tris-HCl (pH 9.0 at 25°C), 50mM NaCl, 5mM MgCl2, 200um each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP), 10 ug activated calf thymus DNA and 0.1mg/ml BSA in a final volume of 50ul.
10X Reaction Buffer with MgCl2
500mM KCl, 100mM Tris-HCl (pH 9.0 at 25C), 1% Triton X-100 and 15mM MgCl2. Buffer is optimized for use with 0.2mM of each dNTP.
10X Reaction Buffer without MgCl2
500mM KCl, 100mM Tris-HCl (pH 9.0 at 25C) and 1% Triton X-100. Include: 10X Reaction Buffer without MgCl2 and separate 25mM MgCl2 Solution.
Features
Depend on an Enzyme That Works: Compositions of the storage buffers have been optimized to assure quality performance of the enzyme under a variety of conditions.
Specify Your Own Reaction Conditions
Choose either Taq with Mg-free 10X Reaction Buffer and separate 25mM MgCl2 or Taq with 10X Reaction Buffer containing 15mM MgCl2
Rely on a Performance-Tested Enzyme
Our PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any of our PCR product, we will send a replacement or refund your account.
Storage Buffer
Compatibility with Reaction Buffers: Taq DNA Polymerase in Storage Buffer. Use of other reaction buffers that do not contain Triton X-100 (final concentration of 0.1%) will result in inactivation of the enzyme.

50mM Tris-HCl (pH 8.0), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 50% glycerol and 1% Triton X-100.

Product Categories/Family for polA recombinant protein

Enzymes; DNA Polymerase

Testing Data(TD) of polA recombinant protein

PCR- amplification of the DNA templates (5ng)with varying lengths from 0.5 to 6.0 kb in a 50 uL reaction volume.

Lane M: 1 kb DNA ladder Marker
Lane 1: 0.5 kb; lane 2: 1 kb, lane 3: 2 kb;
Lane 4: 3 kb; lane 5: 4kb; Lane 6: 6 kb.
polA Recombinant Protein – Cat# MBS142449

Testing Data (TD) of polA recombinant protein

PCR -amplification of a 0.38 kb fragment of human genomic DNA with various amounts of templates (0.05-50 ng) in a a50 ul reaction volume.

Lane 1 : 50 ng template ; Lane 2: 5 ng template; Lane 3: 0.5 ng template
Lane 4: 0.05 ng template; Lane M: DNA Ladder 2000.
polA Recombinant Protein, Testing Data (TD) – Cat# MBS142449

Other Notes

Small volumes of polA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for polA purchase

MBS142449 is a recombinant protein targeting polA. Also known as Recombinant Taq DNA Polymerase. Host: Recombinant E Coli contains Thermus aquaticus polymerase gene.

Additional Details

NCBI/Uniprot data below describe general gene information for polA. It may not necessarily be applicable to this product.

NCBI GI #
118828
NCBI Accession #
P19821.1
UniProt Primary Accession #
P19821
NCBI Official Full Name
DNA polymerase I, thermostable
UniProt Protein Name
DNA polymerase I, thermostable
UniProt Synonym Protein Names
Taq polymerase 1
Protein Family
DNA polymerase
UniProt Gene Name
polA
UniProt Synonym Gene Names
pol1
UniProt Entry Name
DPO1_THEAQ

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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