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Ubiquitin Conjugating Enzyme E2B (UBE2B) Human Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS142481

Description: Ubiquitin Conjugating Enzyme E2B Human Recombinant produced in E Coli is a 19 kDa protein containing 166 amino acids.The UE2B protein contains 6xHis tag and is purified by proprietary chromatographic techniques.Introduction: This E2 enzyme… More details

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Product Overview

Online Datasheet in PDF
Product Name
Ubiquitin Conjugating Enzyme E2B (UBE2B), Recombinant Protein
Full Product Name
Recombinant Human Ubiquitin Conjugating Enzyme E2B
Product Gene Name
UBE2B recombinant protein
Product Synonym Names
UBE2B Human; Ubiquitin Conjugating Enzyme E2B Human Recombinant; Ubiquitin-conjugating enzyme E2 B; EC 6.3.2.19; Ubiquitin-protein ligase B; Ubiquitin carrier protein B; HR6B; hHR6B; E2-17 kDa UBC2; HHR6B; RAD6B; E2-17kDa; UBE2B
Sequence
MHHHHHHAMG QLRSMSTPAR RRLMRDFKRL QEDPPVGVSG APSENNIMQW NAVIFGPEGT PFEDGTFKLV IEFSEEYPNK PPTVRFLSKM FHPNVYADGS ICLDILQNRW SPTYDVSSIL TSIQSLLDEP NPNSPANSQA AQLYQENKRE YEKRVSAIVE QSWNDS
Host
E Coli
Form/Format
Lyophilized from a 0.2um filtered concentrated (1 mg/ml) solution in 1X PBS and 1mM DTT, pH 7.5.
Sterile Filtered white lyophilized powder.
Purity/Purification
Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Preparation and Storage
Lyophilized UBE2B although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution UBE2B should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Molecular Weight
17,312 Da
OMIM
179095
3D Structure
ModBase 3D Structure for P63146
Solubility
It is recommended to reconstitute the lyophilized UBE2B in sterile water not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.

Product Categories/Family for UBE2B recombinant protein

Enzymes; Ubiquitin Conjugating Enzyme

Other Notes

Small volumes of UBE2B recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for UBE2B purchase

MBS142481 is a recombinant protein targeting UBE2B. Also known as Recombinant Human Ubiquitin Conjugating Enzyme E2B. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for UBE2B. It may not necessarily be applicable to this product.

NCBI GI #
4507771
NCBI GeneID
7320
NCBI Accession #
NP_003328.1
UniProt Primary Accession #
P63146
UniProt Secondary Accession #
P23567; Q4PJ15; Q9D0J6; B2R503; D3DQA2
UniProt Related Accession #
P63146
GenBank Nucleotide #
NM_003337.3
NCBI Official Full Name
ubiquitin-conjugating enzyme E2 B
NCBI Official Synonym Full Names
ubiquitin-conjugating enzyme E2B
NCBI Official Symbol
UBE2B
NCBI Official Synonym Symbols
HR6B; UBC2; HHR6B; RAD6B; E2-17kDa
UniProt Protein Name
Ubiquitin-conjugating enzyme E2 B
UniProt Synonym Protein Names
RAD6 homolog B; HR6B; hHR6B; Ubiquitin carrier protein B; Ubiquitin-conjugating enzyme E2-17 kDa; Ubiquitin-protein ligase B
Protein Family
Ubiquitin-conjugating enzyme
UniProt Gene Name
UBE2B
UniProt Synonym Gene Names
RAD6B; HR6B; hHR6B
UniProt Entry Name
UBE2B_HUMAN
NCBI Protein Information
ubiquitin-conjugating enzyme E2 B; E2 protein; RAD6 homolog B; ubiquitin carrier protein B; ubiquitin-conjugating enzyme E2-17 kDa; ubiquitin-conjugating enzyme E2B (RAD6 homolog); ubiquitin-protein ligase B

NCBI Summary for UBE2B

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq, Jul 2008]
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UniProt Comments for UBE2B

UBE2B: a ubiquitin-protein ligase, a member of the ubiquitin-conjugating enzyme family. Homologous to the yeast DNA repair gene RAD6. Interacts with RAD18. Required for postreplication repair of UV-damaged DNA.

Protein type: Ubiquitin ligase; EC 6.3.2.19; Ubiquitin conjugating system; Ligase

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: XY body; cytoplasm; nuclear chromatin; plasma membrane; replication fork; chromatin; nucleus

Molecular Function: protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; response to drug; protein monoubiquitination; proteasomal ubiquitin-dependent protein catabolic process; protein autoubiquitination; protein polyubiquitination; protein stabilization; in utero embryonic development; postreplication repair; protein ubiquitination; Wnt receptor signaling pathway through beta-catenin; DNA repair; negative regulation of histone phosphorylation; cellular response to insulin stimulus; sperm axoneme assembly; response to hypoxia; maintenance of chromatin silencing; spermatogenesis; response to DNA damage stimulus; chiasma formation; histone H2A ubiquitination; response to UV; negative regulation of apoptosis
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Research Articles on UBE2B

1. UBE2B mRNA alterations are associated with severe oligozoospermia in infertile men.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with UBE2B recombinant protein

 Reference Product  PubMed Publications
 UBE2A recombinant protein  >17 publications with UBE2B and UBE2A
 RAD18 recombinant protein  >7 publications with UBE2B and RAD18
 PCNA recombinant protein  >6 publications with UBE2B and PCNA
 UBR2 recombinant protein  >2 publications with UBE2B and UBR2
 UBC recombinant protein  >2 publications with UBE2B and UBC
 RNF20 recombinant protein  >2 publications with UBE2B and RNF20
 RNF40 recombinant protein  >1 publications with UBE2B and RNF40

Pathways associated with UBE2B recombinant protein

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies  Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Proteasome Degradation Pathway antibodies  Proteasome Degradation Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram

Diseases associated with UBE2B recombinant protein

 Disease Name  Pubmed Publications
 Infertility, Male Antibodies  >9 publications with UBE2B and Infertility, Male
 Death Antibodies  >2 publications with UBE2B and Death
 Atrophy Antibodies  >2 publications with UBE2B and Atrophy
 Nervous System Diseases Antibodies  >2 publications with UBE2B and Nervous System Diseases
 Weight Loss Antibodies  >2 publications with UBE2B and Weight Loss
 Lung Diseases Antibodies  >1 publications with UBE2B and Lung Diseases
 Lung Neoplasms Antibodies  >1 publications with UBE2B and Lung Neoplasms
 Cardiovascular Diseases Antibodies  >1 publications with UBE2B and Cardiovascular Diseases
 Hyperplasia Antibodies  >1 publications with UBE2B and Hyperplasia
 Necrosis Antibodies  >1 publications with UBE2B and Necrosis

Organs/Tissues associated with UBE2B recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >7 publications with UBE2B and Testis
 Muscle Antibodies  >3 publications with UBE2B and Muscle
 Lymph Antibodies  >1 publications with UBE2B and Lymph
 Nerve Antibodies  >1 publications with UBE2B and Nerve
 Lymph Node Antibodies  >1 publications with UBE2B and Lymph Node
 Eye Antibodies  >1 publications with UBE2B and Eye
 Blood Antibodies  >1 publications with UBE2B and Blood
 Placenta Antibodies  >1 publications with UBE2B and Placenta
 Adipose Tissue Antibodies  >1 publications with UBE2B and Adipose Tissue
 Lung Antibodies  >1 publications with UBE2B and Lung
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