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Beta Lactamase (LACTB) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS142491

Recombinant E.coli Beta-Lactamase produced in E.Coli is a single, non-glycosylated polypeptide chain containing 264 amino acids and having a molecular mass of approximately 28.9 kDa. Beta Lactamase is purified by proprietary chromatographic techniques. More details

$325 / 1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Beta Lactamase (LACTB), Active Protein
Full Product Name
Recombinant Beta Lactamase
Product Gene Name
Product Synonym Names
b-Lactamase, EC 3.5.2.6, TEM precursor.
Sequence
MHPETLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VEYSPVTEKH LTDGMTVREL CSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DRWEPELNEA IPNDERDTTM PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS RGIIAALGPD GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW.
Form/Format
Lyophilized from a concentrated solution in 100mM Tris, pH7.0
Purity/Purification
Greater than 95.0% as determined by SDS-PAGE.
Preparation and Storage
Lyophilized Beta Lactamase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Beta Lactamase Recombinant should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Source
Escherichia Coli
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Solubility
It is recommended to reconstitute the lyophilized Beta Lactamase in sterile 18MOmega-cm H2O at a concentration of 100 ug/ml, which can then be further diluted to other aqueous solutions. The Beta Lactamase should be used in pH 7.0- 8.0 and in temperature not higher then 45°C.
Biological Activity
One unit will hydrolyze 1.0 umole of benzyl penicillin at pH 7.0 at 25°C, in presence of EDTA.

Product Categories/Family for LACTB active protein

Other Notes

Small volumes of LACTB active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LACTB purchase

Bacteria become resistant to antibodies through poor medical practices and significant help within their genome. Ampicillin resistance is conferred through expression of beta lactamase protein, deactivating the agent. To aid researchers and scientists in the development of novel antimicrob agents chemother, Creative Biomart, a leading producer of recombinant proteins, from influenza virus coats to î² lactamase conserved domain, supplies MyBiosource with DNA RNA sequence verified by basic local alignment tool blast analysis products. This recombinant protein, LACTB, is purified from E coli cell gene expression protein production techniques to _ 90%, making it an ideal choice as + control in assays or arrays, or for generating custom antibody protocols. Our amazing resources, such as Genome Workbench, conserved domain service links, Genomes amp maps and data amp software, have made us #1 as the provider of recombinant beta lactamase TEM precursor. Don't forget to add chemical AMP bioassays to your order.

Additional Details

NCBI/Uniprot data below describe general gene information for LACTB. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
GenBank Nucleotide #
NCBI Official Full Name
beta-lactamase
UniProt Protein Name
Beta-lactamase
UniProt Gene Name
Sbal_2086
UniProt Entry Name
A3D4C4_SHEB5

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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