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Apo Transferrin (TF) Human Native Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS142880

Description: Human Apo Transferrin is a glycoprotein of approximately 77 kDa.Introduction: Transferrin is the iron-transport protein of vertebrate serum and donates iron to cells through interaction with a specific membrane receptor, CD71. Transferrin appears… More details

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Product Overview

Online Datasheet in PDF
Product Name
Apo Transferrin (TF), Native Protein
Full Product Name
Human Apo Transferrin
Product Gene Name
Product Synonym Names
ATF Human; Apo Transferrin Human; Serotransferrin; Transferrin; Siderophilin; Beta-1-metal-binding globulin; TF; PRO1557; PRO2086; DKFZp781D0156; Apo Transferrin; ATF; Apo Transferrin
Form/Format
The protein (10mg/ml) was lyophilized from 20mM NH4HC03 solution. May contain traces of buffer salts.
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity/Purification
Greater than 98.0% as determined by coomassie blue stained SDS-PAGE and Cellulose Acetate electrophoresis.
Preparation and Storage
Lyophilized Apo Transferrin although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Apo Transferrin should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.Please prevent freeze-thaw cycles.
Molecular Weight
77,064 Da
OMIM
190000
3D Structure
ModBase 3D Structure for P02787
Iron Content
The Iron content was estimated by ICP and was found to be less than 4 ppm.
Solubility
It is recommended to reconstitute the lyophilized Apo Transferrin in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Human Virus Test
FDA approved Plasma from each donor has been tested and found negative for antibody to HIV-1, HIV-2, HCV, HBSAG, HBc, ALT and Syphilis. Viral inactivation by pasteurization (60 degree C for 10 hours) has been validated using three different test viruses, with removal of 8-14.5 logs of virus documented. The purification process has also been found to remove significant additional quantities of virus.

Other Notes

Small volumes of TF native protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TF purchase

MBS142880 is a native protein targeting TF. Also known as Human Apo Transferrin.

Additional Details

NCBI/Uniprot data below describe general gene information for TF. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
serotransferrin
NCBI Official Synonym Full Names
transferrin
NCBI Official Symbol
TF
NCBI Official Synonym Symbols
TFQTL1; PRO1557; PRO2086
UniProt Protein Name
Serotransferrin
UniProt Synonym Protein Names
Beta-1 metal-binding globulin; Siderophilin
UniProt Gene Name
TF
UniProt Synonym Gene Names
Transferrin
UniProt Entry Name
TRFE_HUMAN
NCBI Protein Information
serotransferrin; beta-1 metal-binding globulin; siderophilin

NCBI Summary for TF

This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. [provided by RefSeq, Sep 2009]
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UniProt Comments for TF

TF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. Defects in TF are the cause of atransferrinemia (ATRAF). Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. Belongs to the transferrin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: extracellular space; cell surface; cytoplasmic membrane-bound vesicle; early endosome; extracellular region; coated pit; recycling endosome; endocytic vesicle; perinuclear region of cytoplasm; apical plasma membrane; late endosome; basal plasma membrane; endosome membrane; vesicle

Molecular Function: protein binding; ferric iron binding; ubiquitin protein ligase binding

Biological Process: platelet activation; retinal homeostasis; platelet degranulation; cellular iron ion homeostasis; transferrin transport; blood coagulation; transmembrane transport

Disease: Atransferrinemia
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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